10.1371/journal.pone.0232230.g005 Keizo Takenaga Keizo Takenaga Miho Akimoto Miho Akimoto Nobuko Koshikawa Nobuko Koshikawa Hiroki Nagase Hiroki Nagase Infiltration of neutrophils and tumor angiogenesis in orthotopic tumors. Public Library of Science 2020 modulates IL -33-ST mouse pancreatic cancer cell lines mouse pancreatic cancer Panc 02 cells receptor ST 2L pancreatic cancer cells Cxcl 3 expression colon cancer cells CXCL 3 mRNA expression levels CXCL 3-CXCR axis PCR pancreatic cancer sST 2-expressing mouse pancreatic cancer Panc 02 cells tumor microenvironment Conclusions Cancer cell-derived sST 2 Methods ST 2 cancer cell-derived interleukin -33 decoy receptor sST 2 IL -33 decoy receptor sST 2 orthotopic tumor growth sST 2 secretion MPO tumor growth shRNA-resistant sST 2 mRNA sST 2 sST 2 expression ELISA IL -33 knockout mice SB C 57BL mice murine pancreatic cancer model Background Proinflammatory interleukin -33 tumor tissues IL -33-ST 2020-04-27 17:30:02 Figure https://plos.figshare.com/articles/figure/Infiltration_of_neutrophils_and_tumor_angiogenesis_in_orthotopic_tumors_/12201575 <p>(A) The amount of CXCL3 in Panc02-shCont (n = 9) and Panc02-sh#5 (n = 9) tumor tissues. (B) IHC analysis of MPO<sup>+</sup> neutrophil infiltration in Panc02-shCont and Panc02-sh#5 tumors. Bar: 50 μm. (C) The number of MPO<sup>+</sup> cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 2 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 2 tissue sections). (D) RT-qPCR analysis of expression of the neutrophil marker gene <i>Ly6G</i> in Panc02-shCont (n = 5 tumors) and Panc02-sh#5 tumors (n = 6 tumors). (E) The number of F4/80<sup>+</sup> cells per field (×400 magnification) in Panc02-shCont (n = 10 fields of 5 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 4 tissue sections). (F) The number of CD20<sup>+</sup> cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 3 tissue sections) and Panc02-sh#5 tumors (n = 10 fields of 3 tissue sections). (G, H) Angiogenesis in Panc02-shCont and Panc02-sh#5 tumors. G: CD31 staining. Bar: 50 μm. H: Vessel density. Panc02-shCont (n = 11 fields) and Panc02-sh#5 cells (n = 14 fields). The data are shown as the mean ± SD. *P<0.05. ns, not significant.</p>