10.1371/journal.pone.0232230.g005
Keizo Takenaga
Keizo
Takenaga
Miho Akimoto
Miho
Akimoto
Nobuko Koshikawa
Nobuko
Koshikawa
Hiroki Nagase
Hiroki
Nagase
Infiltration of neutrophils and tumor angiogenesis in orthotopic tumors.
Public Library of Science
2020
modulates IL -33-ST
mouse pancreatic cancer cell lines
mouse pancreatic cancer Panc 02 cells
receptor ST 2L
pancreatic cancer cells
Cxcl 3 expression
colon cancer cells
CXCL 3
mRNA expression levels
CXCL 3-CXCR axis
PCR
pancreatic cancer
sST 2-expressing mouse pancreatic cancer Panc 02 cells
tumor microenvironment
Conclusions Cancer cell-derived sST 2
Methods ST 2
cancer cell-derived interleukin -33 decoy receptor sST 2
IL -33 decoy receptor sST 2
orthotopic tumor growth
sST 2 secretion
MPO
tumor growth
shRNA-resistant sST 2 mRNA
sST 2
sST 2 expression
ELISA
IL -33 knockout mice
SB
C 57BL mice
murine pancreatic cancer model Background Proinflammatory interleukin -33
tumor tissues
IL -33-ST
2020-04-27 17:30:02
Figure
https://plos.figshare.com/articles/figure/Infiltration_of_neutrophils_and_tumor_angiogenesis_in_orthotopic_tumors_/12201575
<p>(A) The amount of CXCL3 in Panc02-shCont (n = 9) and Panc02-sh#5 (n = 9) tumor tissues. (B) IHC analysis of MPO<sup>+</sup> neutrophil infiltration in Panc02-shCont and Panc02-sh#5 tumors. Bar: 50 μm. (C) The number of MPO<sup>+</sup> cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 2 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 2 tissue sections). (D) RT-qPCR analysis of expression of the neutrophil marker gene <i>Ly6G</i> in Panc02-shCont (n = 5 tumors) and Panc02-sh#5 tumors (n = 6 tumors). (E) The number of F4/80<sup>+</sup> cells per field (×400 magnification) in Panc02-shCont (n = 10 fields of 5 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 4 tissue sections). (F) The number of CD20<sup>+</sup> cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 3 tissue sections) and Panc02-sh#5 tumors (n = 10 fields of 3 tissue sections). (G, H) Angiogenesis in Panc02-shCont and Panc02-sh#5 tumors. G: CD31 staining. Bar: 50 μm. H: Vessel density. Panc02-shCont (n = 11 fields) and Panc02-sh#5 cells (n = 14 fields). The data are shown as the mean ± SD. *P<0.05. ns, not significant.</p>