mRNA reduction of the crystallins in <i>miak</i> mice at embryonic stages. WadaKenta MatsushimaYoshibumi TadaTomoki HasegawaSayaka ObaraYo YoshizawaYasuhiro TakahashiGou HiaiHiroshi ShimanukiMidori SuzukiSari SaitouJunichi YamamotoNaoki IchikawaMasumi WatanabeKei KikkawaYoshiaki 2014 <p>The relative levels of <i>Cryaa</i>, <i>Cryab</i>, <i>Cryb1</i> and <i>Cryga</i> mRNA in the eye of wild-type (+/+) and <i>miak</i>/<i>miak</i> mice at E11.5, E12.5 and E14.5. The mRNA expression levels were measured by real-time RT-PCR analysis using specific primer sets (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111432#pone.0111432.s004" target="_blank">Table S1</a>) for each gene. The values shown in each graph indicate the mean relative expression levels and the SDs of triplicate eye mRNAs. The expression levels in wild-type mice at E11.5 were assigned an arbitrary value of 1 for comparative purposes. *<i>P<</i>0.05; **<i>P<</i>0.01; ***<i>P<</i>0.001.</p>