Immunoblot and spectroscopy analysis. ChoiAh Reum ShiLichi BrownLeonid S. JungKwang-Hwan 2014 <p>(a) Immunoblot analysis of the <i>Gloeobacter</i> cells and the His-tagged GR protein. The immunoblot used anti-GR antibody for the membrane protein fraction of <i>Gloeobacter</i> cells and anti-His-tag antibody for the protein expressed in <i>E. coli</i>. The whole cells (C) were sonicated and lysates were ultracentrifuged. The lysates were separated into supernatant (A: soluble protein) and pellet (B: membrane protein). D: purified GR from <i>E. coli</i>. (b) Absorption spectra of intact cells and membranes of <i>G. violaceus</i>. The spectrum of <i>G. violaceus</i> is shown with solid line. Chl a (chlorophyll a), Car (carotenoid), PE (phycoerythrin), GR, and PC (phycocyanin) are marked by the arrows. The spectrum of <i>G</i>. <i>violaceus</i> membrane is shown by the dashed line. The spectrum of purified GR in 0.02% DDM, isolated from <i>E. coli</i> is shown by dotted line.</p>