COX-2 knockdown reverses TRAIL-resistance in MCF7 spheroid and monolayer cells. ChandrasekaranSiddarth R. MarshallJocelyn A. MessingJames HsuJong-Wei KingMichael R. 2014 <p>(A) qPCR and western blot data showing the relative expression of COX-2 gene (nā€Š=ā€Š3) and COX-2 protein in control siRNA and COX-2 siRNA transfected MCF7 cells. (B) Flow cytometry histograms and (C) western blot analysis comparing the expression of DR4 in MCF7 cells transfected with control siRNA and COX-2 siRNA cultured as monolayer and spheroids. (D) Bright field images and (E) MTT assay results (nā€Š=ā€Š5) quantifying the effect of 200 ng/mL of TRAIL in control siRNA and COX-2 siRNA transfected cells cultured as monolayer and spheroids.</p>