The COX-2/PGE<sub>2</sub> pathway is upregulated in BT20 and MCF7 spheroids.
Siddarth Chandrasekaran
Jocelyn R. Marshall
James A. Messing
Jong-Wei Hsu
Michael R. King
10.1371/journal.pone.0111487.g005
https://plos.figshare.com/articles/figure/_The_COX_2_PGE_2_pathway_is_upregulated_in_BT20_and_MCF7_spheroids_/1218033
<p>(A) qPCR data showing fold change in COX-2 gene expression in BT20 and MCF7 spheroids in comparison to monolayer cells (nā=ā3) (B) Western blot data indicating the expression of COX-2 protein in whole cell lysates from BT20 and MCF7 cells cultured as spheroids and monolayer (C) ELISA results for PGE<sub>2</sub> level in media conditioned by BT20 and MCF7 cells cultured as spheroids and monolayer (nā=ā3).</p>
2014-10-24 15:25:15
cancer cells
CD 44hiCD population
2 D monolayer
tumor spheroids
breast cancer cells
cox
MCF 7 cells
breast cancer cell lines BT 20
ns
Spheroid cell culture
3 D tumor spheroids
Breast Cancer Cells Cultured
pge
DR 4
CD 44hiCD cells