10.1371/journal.pone.0111487.g005 Siddarth Chandrasekaran Siddarth Chandrasekaran Jocelyn R. Marshall Jocelyn R. Marshall James A. Messing James A. Messing Jong-Wei Hsu Jong-Wei Hsu Michael R. King Michael R. King The COX-2/PGE<sub>2</sub> pathway is upregulated in BT20 and MCF7 spheroids. Public Library of Science 2014 cancer cells CD 44hiCD population 2 D monolayer tumor spheroids breast cancer cells cox MCF 7 cells breast cancer cell lines BT 20 ns Spheroid cell culture 3 D tumor spheroids Breast Cancer Cells Cultured pge DR 4 CD 44hiCD cells 2014-10-24 15:25:15 Figure https://plos.figshare.com/articles/figure/_The_COX_2_PGE_2_pathway_is_upregulated_in_BT20_and_MCF7_spheroids_/1218033 <p>(A) qPCR data showing fold change in COX-2 gene expression in BT20 and MCF7 spheroids in comparison to monolayer cells (nā€Š=ā€Š3) (B) Western blot data indicating the expression of COX-2 protein in whole cell lysates from BT20 and MCF7 cells cultured as spheroids and monolayer (C) ELISA results for PGE<sub>2</sub> level in media conditioned by BT20 and MCF7 cells cultured as spheroids and monolayer (nā€Š=ā€Š3).</p>