10.1371/journal.pgen.1008665.g005 Yoan Arribat Yoan Arribat Dogan Grepper Dogan Grepper Sylviane Lagarrigue Sylviane Lagarrigue Timothy Qi Timothy Qi Sarah Cohen Sarah Cohen Francesca Amati Francesca Amati Wild-type and mutant Spastin affect organelle networks. Public Library of Science 2020 Spastin depletion results show HSP Spastin M 1 Spastin mutations novel biomarkers organelle components system results intracellular dispersion lipid droplet dispersion organelle dynamics Hereditary Spastic Paraplegia MT coordinates LD dispersion Spastin modulates transcripts levels m 1 isoforms splice variants endoplasmic reticulum reticulum Lipid droplets REEP 1. ER shape impacts lipids profile impact LD fate subcellular location Spastin depletion influences LD dispersion impacts LD network 2020-04-21 22:05:50 Figure https://plos.figshare.com/articles/figure/Wild-type_and_mutant_Spastin_affect_organelle_networks_/12170238 <p>Multispectral analysis of organelle size and number in U2OS cells expressing humans Spastin isoforms M1, M1Δex4, M87, M87Δex4 and the mutant M1<sup>H455R</sup>. (A-B) Quantification of lysosome (Lyso) number per cell and median size. (C-D) Quantification of LD number per cell and median size. (E-F) Quantification of peroxisome (Perox) number per cell and median size. (G) Quantification of ER area per cell. (H) Quantification of mitochondria (Mito) area per cell. (I) Quantification of organelle contact area fraction centered on ER. (J) Quantification of organelle contact area fraction centered on LDs. (K-M) Representative images of U2OS cells expressing mock (K), human Spastin M1 (L) and the mutant counterpart M1<sup>H455R</sup> (M). Scale bars, 10 μm. Box whisker plots, statistical tests are unpaired <i>t</i>-tests with Bonferroni correction.</p>