10.1371/journal.pgen.1008665.g005
Yoan Arribat
Yoan
Arribat
Dogan Grepper
Dogan
Grepper
Sylviane Lagarrigue
Sylviane
Lagarrigue
Timothy Qi
Timothy
Qi
Sarah Cohen
Sarah
Cohen
Francesca Amati
Francesca
Amati
Wild-type and mutant Spastin affect organelle networks.
Public Library of Science
2020
Spastin depletion
results show
HSP
Spastin M 1
Spastin mutations
novel biomarkers
organelle components
system results
intracellular dispersion
lipid droplet dispersion
organelle dynamics
Hereditary Spastic Paraplegia
MT
coordinates LD dispersion
Spastin modulates transcripts levels
m 1 isoforms
splice variants
endoplasmic reticulum
reticulum Lipid droplets
REEP 1.
ER shape
impacts lipids profile
impact LD fate
subcellular location
Spastin depletion influences
LD dispersion
impacts LD network
2020-04-21 22:05:50
Figure
https://plos.figshare.com/articles/figure/Wild-type_and_mutant_Spastin_affect_organelle_networks_/12170238
<p>Multispectral analysis of organelle size and number in U2OS cells expressing humans Spastin isoforms M1, M1Δex4, M87, M87Δex4 and the mutant M1<sup>H455R</sup>. (A-B) Quantification of lysosome (Lyso) number per cell and median size. (C-D) Quantification of LD number per cell and median size. (E-F) Quantification of peroxisome (Perox) number per cell and median size. (G) Quantification of ER area per cell. (H) Quantification of mitochondria (Mito) area per cell. (I) Quantification of organelle contact area fraction centered on ER. (J) Quantification of organelle contact area fraction centered on LDs. (K-M) Representative images of U2OS cells expressing mock (K), human Spastin M1 (L) and the mutant counterpart M1<sup>H455R</sup> (M). Scale bars, 10 μm. Box whisker plots, statistical tests are unpaired <i>t</i>-tests with Bonferroni correction.</p>