Arribat, Yoan Grepper, Dogan Lagarrigue, Sylviane Qi, Timothy Cohen, Sarah Amati, Francesca Spastin splice variants in zebrafish. <p>(A) Spastin phylogenic tree (designed from <a href="http://www.phylogeny.fr/" target="_blank">www.phylogeny.fr</a> –Substitution model: Dayhoff). (B) Whole-mount <i>in situ</i> hybridization of Spastin transcript in zebrafish embryos (at 14, 18, 28, 72 hpf). Antisense probe (AS) correspond to Spastin transcript. Sense probe (S) to negative control. n = 10 embryos for each group. (C) RT-PCR amplifying different regions of Spastin from embryonic and adult zebrafish cDNA. (D) Schematic representation of Spastin splice variants in zebrafish. (E) Confocal microscopy images of zebrafish embryonic cells overexpressing Spastin splice variants (treated with 300μM oleic acid for 18h before acquisition). Top right insets correspond to higher magnification centered on LDs. Cherry-tagged Spastin appears in red. Tubulin labeling corresponds to microtubules (cyan), Bodipy to LDs (yellow) and Hoechst to nucleus (blue). N = 4 experiments. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008665#pgen.1008665.s001" target="_blank">S1 Fig</a>.</p> Spastin depletion;results show;HSP;Spastin M 1;Spastin mutations;novel biomarkers;organelle components;system results;intracellular dispersion;lipid droplet dispersion;organelle dynamics;Hereditary Spastic Paraplegia;MT;coordinates LD dispersion;Spastin modulates transcripts levels;m 1 isoforms;splice variants;endoplasmic reticulum;reticulum Lipid droplets;REEP 1.;ER shape;impacts lipids profile;impact LD fate;subcellular location;Spastin depletion influences;LD dispersion;impacts LD network 2020-04-21
    https://plos.figshare.com/articles/figure/Spastin_splice_variants_in_zebrafish_/12170223
10.1371/journal.pgen.1008665.g001