10.1371/journal.pgen.1008665.g001
Yoan Arribat
Yoan
Arribat
Dogan Grepper
Dogan
Grepper
Sylviane Lagarrigue
Sylviane
Lagarrigue
Timothy Qi
Timothy
Qi
Sarah Cohen
Sarah
Cohen
Francesca Amati
Francesca
Amati
Spastin splice variants in zebrafish.
Public Library of Science
2020
Spastin depletion
results show
HSP
Spastin M 1
Spastin mutations
novel biomarkers
organelle components
system results
intracellular dispersion
lipid droplet dispersion
organelle dynamics
Hereditary Spastic Paraplegia
MT
coordinates LD dispersion
Spastin modulates transcripts levels
m 1 isoforms
splice variants
endoplasmic reticulum
reticulum Lipid droplets
REEP 1.
ER shape
impacts lipids profile
impact LD fate
subcellular location
Spastin depletion influences
LD dispersion
impacts LD network
2020-04-21 22:05:45
Figure
https://plos.figshare.com/articles/figure/Spastin_splice_variants_in_zebrafish_/12170223
<p>(A) Spastin phylogenic tree (designed from <a href="http://www.phylogeny.fr/" target="_blank">www.phylogeny.fr</a> –Substitution model: Dayhoff). (B) Whole-mount <i>in situ</i> hybridization of Spastin transcript in zebrafish embryos (at 14, 18, 28, 72 hpf). Antisense probe (AS) correspond to Spastin transcript. Sense probe (S) to negative control. n = 10 embryos for each group. (C) RT-PCR amplifying different regions of Spastin from embryonic and adult zebrafish cDNA. (D) Schematic representation of Spastin splice variants in zebrafish. (E) Confocal microscopy images of zebrafish embryonic cells overexpressing Spastin splice variants (treated with 300μM oleic acid for 18h before acquisition). Top right insets correspond to higher magnification centered on LDs. Cherry-tagged Spastin appears in red. Tubulin labeling corresponds to microtubules (cyan), Bodipy to LDs (yellow) and Hoechst to nucleus (blue). N = 4 experiments. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008665#pgen.1008665.s001" target="_blank">S1 Fig</a>.</p>