10.1371/journal.pgen.1008665.g001 Yoan Arribat Yoan Arribat Dogan Grepper Dogan Grepper Sylviane Lagarrigue Sylviane Lagarrigue Timothy Qi Timothy Qi Sarah Cohen Sarah Cohen Francesca Amati Francesca Amati Spastin splice variants in zebrafish. Public Library of Science 2020 Spastin depletion results show HSP Spastin M 1 Spastin mutations novel biomarkers organelle components system results intracellular dispersion lipid droplet dispersion organelle dynamics Hereditary Spastic Paraplegia MT coordinates LD dispersion Spastin modulates transcripts levels m 1 isoforms splice variants endoplasmic reticulum reticulum Lipid droplets REEP 1. ER shape impacts lipids profile impact LD fate subcellular location Spastin depletion influences LD dispersion impacts LD network 2020-04-21 22:05:45 Figure https://plos.figshare.com/articles/figure/Spastin_splice_variants_in_zebrafish_/12170223 <p>(A) Spastin phylogenic tree (designed from <a href="http://www.phylogeny.fr/" target="_blank">www.phylogeny.fr</a> –Substitution model: Dayhoff). (B) Whole-mount <i>in situ</i> hybridization of Spastin transcript in zebrafish embryos (at 14, 18, 28, 72 hpf). Antisense probe (AS) correspond to Spastin transcript. Sense probe (S) to negative control. n = 10 embryos for each group. (C) RT-PCR amplifying different regions of Spastin from embryonic and adult zebrafish cDNA. (D) Schematic representation of Spastin splice variants in zebrafish. (E) Confocal microscopy images of zebrafish embryonic cells overexpressing Spastin splice variants (treated with 300μM oleic acid for 18h before acquisition). Top right insets correspond to higher magnification centered on LDs. Cherry-tagged Spastin appears in red. Tubulin labeling corresponds to microtubules (cyan), Bodipy to LDs (yellow) and Hoechst to nucleus (blue). N = 4 experiments. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008665#pgen.1008665.s001" target="_blank">S1 Fig</a>.</p>