10.1371/journal.pgen.1008676.g005 Norio Hamada Norio Hamada Nobuhiko Hamazaki Nobuhiko Hamazaki So Shimamoto So Shimamoto Orie Hikabe Orie Hikabe Go Nagamatsu Go Nagamatsu Yuki Takada Yuki Takada Kiyoko Kato Kiyoko Kato Katsuhiko Hayashi Katsuhiko Hayashi Inhibitory effect of <i>Eif2s3y</i> on oogenesis. Public Library of Science 2020 culture system sex chromosomes Germ cell-intrinsic effects germ cell-intrinsic effect gametogenesi sex chromosome disorders oogenesi XO novel culture system XX XY ESCs 2020-03-26 17:44:39 Figure https://plos.figshare.com/articles/figure/Inhibitory_effect_of_i_Eif2s3y_i_on_oogenesis_/12037143 <p>(<b>A</b>) Expression level of Y-linked genes in XY oocytes at agg-7. Black bars indicate SD. (<b>B</b>) Expression dynamics of <i>Eif2s3y</i>. The graph shows TPM and SD of <i>Eif2s3y</i> in XX and XY cells. (<b>C</b>) Oocyte differentiation from <i>Eif2s3y</i>-expressing XX and XO ESCs. Images are rOvaries at agg-21. The expression level of <i>Eif2s3y</i> is shown as high, middle or low, based on <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008676#pgen.1008676.s006" target="_blank">S6B Fig</a>. Scale bars, 200 μm. (<b>D</b>) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes formed in each genotype. <i>P</i> values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant. (<b>E</b>) Expression dynamics of <i>Eif2s3x</i>. The graph shows TPM and SD of <i>Eif2s3y</i> in XX and XY cells. (<b>F</b>) Total amount of <i>Eif2s3x</i> and <i>Eif2s3y</i> transcripts. (<b>G</b>) Oocyte differentiation from <i>Eif2s3x</i>-expressing XX ESCs. Images are rOvaries at agg-21. The expression level of <i>Eif2s3x</i> is shown as high or middle, based on <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008676#pgen.1008676.s006" target="_blank">S6B Fig</a>. Scale bars, 200 μm. (H) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes formed in each genotype. <i>P</i> values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant.</p>