10.1371/journal.pgen.1008676.g005
Norio Hamada
Norio
Hamada
Nobuhiko Hamazaki
Nobuhiko
Hamazaki
So Shimamoto
So
Shimamoto
Orie Hikabe
Orie
Hikabe
Go Nagamatsu
Go
Nagamatsu
Yuki Takada
Yuki
Takada
Kiyoko Kato
Kiyoko
Kato
Katsuhiko Hayashi
Katsuhiko
Hayashi
Inhibitory effect of <i>Eif2s3y</i> on oogenesis.
Public Library of Science
2020
culture system
sex chromosomes
Germ cell-intrinsic effects
germ cell-intrinsic effect
gametogenesi
sex chromosome disorders
oogenesi
XO
novel culture system
XX
XY ESCs
2020-03-26 17:44:39
Figure
https://plos.figshare.com/articles/figure/Inhibitory_effect_of_i_Eif2s3y_i_on_oogenesis_/12037143
<p>(<b>A</b>) Expression level of Y-linked genes in XY oocytes at agg-7. Black bars indicate SD. (<b>B</b>) Expression dynamics of <i>Eif2s3y</i>. The graph shows TPM and SD of <i>Eif2s3y</i> in XX and XY cells. (<b>C</b>) Oocyte differentiation from <i>Eif2s3y</i>-expressing XX and XO ESCs. Images are rOvaries at agg-21. The expression level of <i>Eif2s3y</i> is shown as high, middle or low, based on <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008676#pgen.1008676.s006" target="_blank">S6B Fig</a>. Scale bars, 200 μm. (<b>D</b>) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes formed in each genotype. <i>P</i> values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant. (<b>E</b>) Expression dynamics of <i>Eif2s3x</i>. The graph shows TPM and SD of <i>Eif2s3y</i> in XX and XY cells. (<b>F</b>) Total amount of <i>Eif2s3x</i> and <i>Eif2s3y</i> transcripts. (<b>G</b>) Oocyte differentiation from <i>Eif2s3x</i>-expressing XX ESCs. Images are rOvaries at agg-21. The expression level of <i>Eif2s3x</i> is shown as high or middle, based on <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008676#pgen.1008676.s006" target="_blank">S6B Fig</a>. Scale bars, 200 μm. (H) The number of oocytes formed in culture. Each dot indicates the number of oocytes formed in one rOvary. The numbers in the graph indicate the average number of oocytes formed in each genotype. <i>P</i> values were calculated by Steel Dwass test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant.</p>