Hamada, Norio Hamazaki, Nobuhiko Shimamoto, So Hikabe, Orie Nagamatsu, Go Takada, Yuki Kato, Kiyoko Hayashi, Katsuhiko Meiotic initiation and progression in XX, XO and XY PGCLC/oocytes. <p>(<b>A</b>) Meiotic initiation in XX, XO and XY PGCLC/oocytes. Images show the immunofluorescence analysis of SYCP3 (green) and NANOG (red) in PGCLCs/oocytes at agg-7. Scale bars, 50 μm. (<b>B</b>) Time-course of meiotic entry. The graph shows the average percentage and SD of SYCP3- or NANOG-positive cells at the days indicated. The data were compiled from two independent experiments. (<b>C</b>) EdU incorporation into NANOG-positive cells. Images show the immunofluorescence analysis of BV/SC (green), NANOG (red), EdU (Blue) and their merged images in rOvaries. The number below the images indicates NANOG-positive cells per EdU-positive cells. Scale bars, 50 μm. (<b>D</b>) The percentage of EdU-positive cells per BV- and/or SC-positive cells. The graph indicates the average percentage and SD of EdU-positive cells per BV- and/or SC-positive cells. The data were compiled from two independent experiments. (<b>E</b>) Schematic diagram of the reconstitution experiment using PGCs derived in vivo. XX or XY PGCs of E10.5 embryos were reaggregated with gonadal somatic cells of E12.5 female embryos. (<b>F</b>) The percentage of cells entering meiosis. The graph shows the average percentage and SD of SYCP3- or NANOG-positive cells at the days indicated. The data was compiled from four independent experiments. <i>P</i> values were calculated by <i>t</i>-test. ***P<0.001. (<b>G</b>) NANOG-positive XY germ cells in the rOvary. Images show the immunofluorescence analysis of SYCP3 (green), NANOG (red), TRA98 (a marker of germ cells: white) and their merged images. The dashed lines indicate representative NANOG-positive germ cells. Scale bars, 50 μm.</p> culture system;sex chromosomes;Germ cell-intrinsic effects;germ cell-intrinsic effect;gametogenesi;sex chromosome disorders;oogenesi;XO;novel culture system;XX;XY ESCs 2020-03-26
    https://plos.figshare.com/articles/figure/Meiotic_initiation_and_progression_in_XX_XO_and_XY_PGCLC_oocytes_/12037137
10.1371/journal.pgen.1008676.g003