Comparing the viability quantified with fluorescence assays and microscopy.
Laura Braun
Lucinda Hazell
Alexander J. Webb
Fiona Allan
Aidan M. Emery
Michael R. Templeton
10.1371/journal.pntd.0008176.g007
https://plos.figshare.com/articles/figure/Comparing_the_viability_quantified_with_fluorescence_assays_and_microscopy_/12037128
<p>Samples containing 100 ± 4 cercariae with a viability of 30% ± 2% were stained with FDA-PI and FDA-H. Measurements were taken at 20 minutes. Three 50 μl aliquots of each sample were then examined under the microscope, and cercarial viability was recorded by counting moving (viable) and non-moving (non-viable) cercariae. Average quantified viabilities and SEMs are shown on the bar graph. The “actual” viability is the known proportion of live and dead cercariae in each well, chosen as 30%.</p>
2020-03-26 17:44:31
water treatment processes
microscopy
FDA
method
Natural History Museum
Examining cercarial movement
water treatment Background Schistosome cercariae
quantifying cercarial viability
fluorescence assays
Schistosoma mansoni cercariae
water samples