Comparing the viability quantified with fluorescence assays and microscopy. Laura Braun Lucinda Hazell Alexander J. Webb Fiona Allan Aidan M. Emery Michael R. Templeton 10.1371/journal.pntd.0008176.g007 https://plos.figshare.com/articles/figure/Comparing_the_viability_quantified_with_fluorescence_assays_and_microscopy_/12037128 <p>Samples containing 100 ± 4 cercariae with a viability of 30% ± 2% were stained with FDA-PI and FDA-H. Measurements were taken at 20 minutes. Three 50 μl aliquots of each sample were then examined under the microscope, and cercarial viability was recorded by counting moving (viable) and non-moving (non-viable) cercariae. Average quantified viabilities and SEMs are shown on the bar graph. The “actual” viability is the known proportion of live and dead cercariae in each well, chosen as 30%.</p> 2020-03-26 17:44:31 water treatment processes microscopy FDA method Natural History Museum Examining cercarial movement water treatment Background Schistosome cercariae quantifying cercarial viability fluorescence assays Schistosoma mansoni cercariae water samples