Moreira, Beatriz Lourenço Correia Gimenez, Ana Paula Lappas Inagaki, Jorge Minor Fernandes Raboni, Sonia Mara Confirmation of infectivity <i>in vivo</i>. <p>Note: (A) mice injected intra-cerebrally with IVS–, 21 days after inoculation showing no clinical signs of rabies; (B) mice injected intra-cerebrally with IVS+, 5 days after inoculation showing specific rabies symptoms, such as ruffled fur, hunched back, slow movements and paralysis; (C) brain DFA of mouse injected intra-cerebrally with IVS–(40x); (D) brain DFA of mouse injected intra-cerebrally with IVS+ (40x); (E) brain TEM of mouse inoculated with IVS–(1 μm); (F) brain TEM of mouse inoculated with IVS+, arrow indicates rabies virus particles (200 nm); (G) brain H&E stain of mouse inoculated with IVS–, basal ganglia with normal cellularity (50x); (H) brain H&E stain of mouse inoculated with IVS+, Purkinje cell with Negri body–arrow (400x).</p> laboratory animals numbers;quality control;vivo quality control trials;virus detection Human rabies;method;assay;Inactivated rabies vaccines;rabies virus particles;rabies vaccine production;quality control schemes 2020-03-25
    https://plos.figshare.com/articles/figure/Confirmation_of_infectivity_i_in_vivo_i_/12032049
10.1371/journal.pntd.0008142.g007