%0 Figure %A C. Rocha, Sayonarah %A T. C. Pessoa, Marco %A D. R. Neves, Luiza %A L. G. Alves, Silmara %A Silva, Luciana M. %A L. Santos, Herica %A M. F. Oliveira, Soraya %A G. Taranto, Alex %A Comar, Moacyr %A V. Gomes, Isabella %A V. Santos, Fabio %A Paixão, Natasha %A E. M. Quintas, Luis %A Noël, François %A F. Pereira, Antonio %A C. S. C. Tessis, Ana %A L. S. Gomes, Natalia %A C. Moreira, Otacilio %A Rincon-Heredia, Ruth %A P. Varotti, Fernando %A Blanco, Gustavo %A A. F. P. Villar, Jose %A G. Contreras, Rubén %A A. Barbosa, Leandro %D 2014 %T 21-BD induces apoptosis in HeLa and CHO-K1 cells. %U https://plos.figshare.com/articles/figure/_21_BD_induces_apoptosis_in_HeLa_and_CHO_K1_cells_/1196751 %R 10.1371/journal.pone.0108776.g005 %2 https://plos.figshare.com/ndownloader/files/1708457 %K bd %K Junctions Cardiotonic steroids %K RKO cancer cells %K na %K cardiotonic %K modulates epithelial cell adhesion %K db %K multidrug exporter Pdr 5p %K digoxin %K epithelial MDCK cells %K lactone ring %X

(A) Score value obtained from the comet assay of CHO-K1 cells incubated 24 h with 21-BD at different concentrations (red circles). (B) Micronucleated cells percentage of CHO-K1 cultures incubated with 21-BD at different concentrations for 24 h. A 24 h incubation with 0.4 mM Methyl methanesulfonate (MMS) was used as a control (A, B, blue circles). (C) Apoptotic and necrotic HeLa cells after 24 h of incubation in control media (white bars), media with 50 µM 21-BD (red bars) or 2 µM digoxin (green bars) for 24 h. Apoptosis and necrosis were detected by flow cytometry ussing an annexin-V translocation assay and the incorporation of propidium iodide in to the nucleus, respectively. P<0.01.

%I PLOS ONE