10.1371/journal.pone.0108776.g005
Sayonarah C. Rocha
Sayonarah
C. Rocha
Marco T. C. Pessoa
Marco
T. C. Pessoa
Luiza D. R. Neves
Luiza
D. R. Neves
Silmara L. G. Alves
Silmara
L. G. Alves
Luciana M. Silva
Luciana M.
Silva
Herica L. Santos
Herica
L. Santos
Soraya M. F. Oliveira
Soraya
M. F. Oliveira
Alex G. Taranto
Alex
G. Taranto
Moacyr Comar
Moacyr
Comar
Isabella V. Gomes
Isabella
V. Gomes
Fabio V. Santos
Fabio
V. Santos
Natasha Paixão
Natasha
Paixão
Luis E. M. Quintas
Luis
E. M. Quintas
François Noël
François
Noël
Antonio F. Pereira
Antonio
F. Pereira
Ana C. S. C. Tessis
Ana
C. S. C. Tessis
Natalia L. S. Gomes
Natalia
L. S. Gomes
Otacilio C. Moreira
Otacilio
C. Moreira
Ruth Rincon-Heredia
Ruth
Rincon-Heredia
Fernando P. Varotti
Fernando
P. Varotti
Gustavo Blanco
Gustavo
Blanco
Jose A. F. P. Villar
Jose
A. F. P. Villar
Rubén G. Contreras
Rubén
G. Contreras
Leandro A. Barbosa
Leandro
A. Barbosa
21-BD induces apoptosis in HeLa and CHO-K1 cells.
Public Library of Science
2014
bd
Junctions Cardiotonic steroids
RKO cancer cells
na
cardiotonic
modulates epithelial cell adhesion
db
multidrug exporter Pdr 5p
digoxin
epithelial MDCK cells
lactone ring
2014-10-07 03:18:49
Figure
https://plos.figshare.com/articles/figure/_21_BD_induces_apoptosis_in_HeLa_and_CHO_K1_cells_/1196751
<p>(A) Score value obtained from the comet assay of CHO-K1 cells incubated 24 h with 21-BD at different concentrations (red circles). (B) Micronucleated cells percentage of CHO-K1 cultures incubated with 21-BD at different concentrations for 24 h. A 24 h incubation with 0.4 mM Methyl methanesulfonate (MMS) was used as a control (A, B, blue circles). (C) Apoptotic and necrotic HeLa cells after 24 h of incubation in control media (white bars), media with 50 µM 21-BD (red bars) or 2 µM digoxin (green bars) for 24 h. Apoptosis and necrosis were detected by flow cytometry ussing an annexin-V translocation assay and the incorporation of propidium iodide in to the nucleus, respectively. <i>P</i><0.01.</p>