10.1371/journal.pone.0108776.g005 Sayonarah C. Rocha Sayonarah C. Rocha Marco T. C. Pessoa Marco T. C. Pessoa Luiza D. R. Neves Luiza D. R. Neves Silmara L. G. Alves Silmara L. G. Alves Luciana M. Silva Luciana M. Silva Herica L. Santos Herica L. Santos Soraya M. F. Oliveira Soraya M. F. Oliveira Alex G. Taranto Alex G. Taranto Moacyr Comar Moacyr Comar Isabella V. Gomes Isabella V. Gomes Fabio V. Santos Fabio V. Santos Natasha Paixão Natasha Paixão Luis E. M. Quintas Luis E. M. Quintas François Noël François Noël Antonio F. Pereira Antonio F. Pereira Ana C. S. C. Tessis Ana C. S. C. Tessis Natalia L. S. Gomes Natalia L. S. Gomes Otacilio C. Moreira Otacilio C. Moreira Ruth Rincon-Heredia Ruth Rincon-Heredia Fernando P. Varotti Fernando P. Varotti Gustavo Blanco Gustavo Blanco Jose A. F. P. Villar Jose A. F. P. Villar Rubén G. Contreras Rubén G. Contreras Leandro A. Barbosa Leandro A. Barbosa 21-BD induces apoptosis in HeLa and CHO-K1 cells. Public Library of Science 2014 bd Junctions Cardiotonic steroids RKO cancer cells na cardiotonic modulates epithelial cell adhesion db multidrug exporter Pdr 5p digoxin epithelial MDCK cells lactone ring 2014-10-07 03:18:49 Figure https://plos.figshare.com/articles/figure/_21_BD_induces_apoptosis_in_HeLa_and_CHO_K1_cells_/1196751 <p>(A) Score value obtained from the comet assay of CHO-K1 cells incubated 24 h with 21-BD at different concentrations (red circles). (B) Micronucleated cells percentage of CHO-K1 cultures incubated with 21-BD at different concentrations for 24 h. A 24 h incubation with 0.4 mM Methyl methanesulfonate (MMS) was used as a control (A, B, blue circles). (C) Apoptotic and necrotic HeLa cells after 24 h of incubation in control media (white bars), media with 50 µM 21-BD (red bars) or 2 µM digoxin (green bars) for 24 h. Apoptosis and necrosis were detected by flow cytometry ussing an annexin-V translocation assay and the incorporation of propidium iodide in to the nucleus, respectively. <i>P</i><0.01.</p>