10.1371/journal.pone.0108776.g004 Sayonarah C. Rocha Sayonarah C. Rocha Marco T. C. Pessoa Marco T. C. Pessoa Luiza D. R. Neves Luiza D. R. Neves Silmara L. G. Alves Silmara L. G. Alves Luciana M. Silva Luciana M. Silva Herica L. Santos Herica L. Santos Soraya M. F. Oliveira Soraya M. F. Oliveira Alex G. Taranto Alex G. Taranto Moacyr Comar Moacyr Comar Isabella V. Gomes Isabella V. Gomes Fabio V. Santos Fabio V. Santos Natasha Paixão Natasha Paixão Luis E. M. Quintas Luis E. M. Quintas François Noël François Noël Antonio F. Pereira Antonio F. Pereira Ana C. S. C. Tessis Ana C. S. C. Tessis Natalia L. S. Gomes Natalia L. S. Gomes Otacilio C. Moreira Otacilio C. Moreira Ruth Rincon-Heredia Ruth Rincon-Heredia Fernando P. Varotti Fernando P. Varotti Gustavo Blanco Gustavo Blanco Jose A. F. P. Villar Jose A. F. P. Villar Rubén G. Contreras Rubén G. Contreras Leandro A. Barbosa Leandro A. Barbosa High concentrations of 21-BD reduce cell viability of HeLa and RKO. Public Library of Science 2014 bd Junctions Cardiotonic steroids RKO cancer cells na cardiotonic modulates epithelial cell adhesion db multidrug exporter Pdr 5p digoxin epithelial MDCK cells lactone ring 2014-10-07 03:18:49 Figure https://plos.figshare.com/articles/figure/_High_concentrations_of_21_BD_reduce_cell_viability_of_HeLa_and_RKO_/1196750 <p>HeLa (A) or RKO (B) cells were treated with digoxin (green symbols) or 21-BD (red symbols) for 24 (circles) or 48 (squares) h. Viability was measured by MTT reduction assay. 100 and 25 µM 21-BD induced the statistically significant reduction of HeLa and RKO viability, respectively (p<0.0084). Digoxin reduces HeLa viability starting with 150 µM for 24 h and 50 µM for 48 h (p<0.001). RKO cells have a higher sensitivity to digoxin that induces statistically significant differences starting from 1.6 µM for 48 h (p<0.0001).</p>