10.1371/journal.pone.0108776.g002
Sayonarah C. Rocha
Sayonarah
C. Rocha
Marco T. C. Pessoa
Marco
T. C. Pessoa
Luiza D. R. Neves
Luiza
D. R. Neves
Silmara L. G. Alves
Silmara
L. G. Alves
Luciana M. Silva
Luciana M.
Silva
Herica L. Santos
Herica
L. Santos
Soraya M. F. Oliveira
Soraya
M. F. Oliveira
Alex G. Taranto
Alex
G. Taranto
Moacyr Comar
Moacyr
Comar
Isabella V. Gomes
Isabella
V. Gomes
Fabio V. Santos
Fabio
V. Santos
Natasha Paixão
Natasha
Paixão
Luis E. M. Quintas
Luis
E. M. Quintas
François Noël
François
Noël
Antonio F. Pereira
Antonio
F. Pereira
Ana C. S. C. Tessis
Ana
C. S. C. Tessis
Natalia L. S. Gomes
Natalia
L. S. Gomes
Otacilio C. Moreira
Otacilio
C. Moreira
Ruth Rincon-Heredia
Ruth
Rincon-Heredia
Fernando P. Varotti
Fernando
P. Varotti
Gustavo Blanco
Gustavo
Blanco
Jose A. F. P. Villar
Jose
A. F. P. Villar
Rubén G. Contreras
Rubén
G. Contreras
Leandro A. Barbosa
Leandro
A. Barbosa
21-BD effect on Na,K-ATPase and Pdr5p activity.
Public Library of Science
2014
bd
Junctions Cardiotonic steroids
RKO cancer cells
na
cardiotonic
modulates epithelial cell adhesion
db
multidrug exporter Pdr 5p
digoxin
epithelial MDCK cells
lactone ring
2014-10-07 03:18:49
Figure
https://plos.figshare.com/articles/figure/_21_BD_effect_on_Na_K_ATPase_and_Pdr5p_activity_/1196746
<p>(A) 21-BD competition of <sup>3</sup>H-ouabain binding on HeLa cells; the control for maximal binding is represented with a white circle and a long dashed line, competition of ouabain and 21-BD is shown with blue and red circles respectively. (B) Inhibition of rat´s brain hemisphere Na,K-ATPase after 2 h incubation with digoxin (green circles) or 21-BD (red circles). (C) Effect of 21-BD on the Na,K-ATPase activity on proteins expressed in Sf9 insect cells, Na,K-ATPase activity was measured on Sf9 cells expressing the rat α<sub>1</sub> β<sub>1</sub> (orange circles) or β<sub>1</sub> (red circles) after 15 min treatment with the indicated concentrations of 21-BD. (D) Dose-response curve for the effects of 21-DB on Na,K-ATPase activity of mouse kidney membrane preparations. E) Effect of 21-BD (red circles) or digoxin (green circles) on the activity of the Pdr5p transporter.</p>