10.1371/journal.pone.0108776.g002 Sayonarah C. Rocha Sayonarah C. Rocha Marco T. C. Pessoa Marco T. C. Pessoa Luiza D. R. Neves Luiza D. R. Neves Silmara L. G. Alves Silmara L. G. Alves Luciana M. Silva Luciana M. Silva Herica L. Santos Herica L. Santos Soraya M. F. Oliveira Soraya M. F. Oliveira Alex G. Taranto Alex G. Taranto Moacyr Comar Moacyr Comar Isabella V. Gomes Isabella V. Gomes Fabio V. Santos Fabio V. Santos Natasha Paixão Natasha Paixão Luis E. M. Quintas Luis E. M. Quintas François Noël François Noël Antonio F. Pereira Antonio F. Pereira Ana C. S. C. Tessis Ana C. S. C. Tessis Natalia L. S. Gomes Natalia L. S. Gomes Otacilio C. Moreira Otacilio C. Moreira Ruth Rincon-Heredia Ruth Rincon-Heredia Fernando P. Varotti Fernando P. Varotti Gustavo Blanco Gustavo Blanco Jose A. F. P. Villar Jose A. F. P. Villar Rubén G. Contreras Rubén G. Contreras Leandro A. Barbosa Leandro A. Barbosa 21-BD effect on Na,K-ATPase and Pdr5p activity. Public Library of Science 2014 bd Junctions Cardiotonic steroids RKO cancer cells na cardiotonic modulates epithelial cell adhesion db multidrug exporter Pdr 5p digoxin epithelial MDCK cells lactone ring 2014-10-07 03:18:49 Figure https://plos.figshare.com/articles/figure/_21_BD_effect_on_Na_K_ATPase_and_Pdr5p_activity_/1196746 <p>(A) 21-BD competition of <sup>3</sup>H-ouabain binding on HeLa cells; the control for maximal binding is represented with a white circle and a long dashed line, competition of ouabain and 21-BD is shown with blue and red circles respectively. (B) Inhibition of rat´s brain hemisphere Na,K-ATPase after 2 h incubation with digoxin (green circles) or 21-BD (red circles). (C) Effect of 21-BD on the Na,K-ATPase activity on proteins expressed in Sf9 insect cells, Na,K-ATPase activity was measured on Sf9 cells expressing the rat α<sub>1</sub> β<sub>1</sub> (orange circles) or β<sub>1</sub> (red circles) after 15 min treatment with the indicated concentrations of 21-BD. (D) Dose-response curve for the effects of 21-DB on Na,K-ATPase activity of mouse kidney membrane preparations. E) Effect of 21-BD (red circles) or digoxin (green circles) on the activity of the Pdr5p transporter.</p>