10.1371/journal.ppat.1004358.g002 Yue Qin Yue Qin Mao-Tian Zhou Mao-Tian Zhou Ming-Ming Hu Ming-Ming Hu Yun-Hong Hu Yun-Hong Hu Jing Zhang Jing Zhang Lin Guo Lin Guo Bo Zhong Bo Zhong Hong-Bing Shu Hong-Bing Shu RNF26 interacts with MITA. Public Library of Science 2014 RING finger protein 26 E 3 ubiquitin ligase proinflammatory cytokine induction polyubiquitination sting Distinct Mechanisms Viral infection triggers induction IFNB 1 gene RNF 26 antagonizes RNF 26 mita response IRF 3 activation 2014-09-25 04:01:43 Figure https://plos.figshare.com/articles/figure/_RNF26_interacts_with_MITA_/1183088 <p>(A) RNF26 interacted with MITA. The 293 cells (5×10<sup>6</sup>) were transfected with the indicated plasmids (5 µg each). Twenty-four hours later, cells were lysed and cell lysates were immunoprecipitated with anti-Flag or control IgG. The immunoprecipitates and whole cell lysates were analyzed by immunoblots with anti-HA or anti-Flag. (B) RNF26 was physiologically associated with MITA. THP-1 cells (2×10<sup>7</sup>) were infected with SeV or HSV-1 for the indicated time points or left uninfected. Cells were lysed and immunoprecipitation and immunoblot analysis was performed with antibodies against the indicated proteins. NC, negative control. (C) RNF26 is localized to the ER and mitochondria. HeLa cells (2×10<sup>5</sup>) were transfected with the indicated plasmids (0.5 µg each). Twenty-four hours after transfection, the cells were stained with Mito-Tracker Red for 15 minutes or left untreated. The cells were fixed with 4% paraformaldehyde and subjected for confocal microscopy analysis. (D) Subcellular distribution of RNF26. The 293 cells (5×10<sup>7</sup>) were infected with SeV for the indicated time points or left uninfected. Subcellular fractionation assays were performed and the cellular fractions were analyzed by immunoblots with antibodies against the indicated proteins. (E) RNF26 and MITA were colocalized to the ER. HeLa cells (2×10<sup>5</sup>) were transfected with the indicated plasmids (0.5 µg each). Eighteen hours after transfection, cells were infected with SeV or HSV-1 for 6 hours or left uninfected, followed by ER-Tracker Blue/White staining for 30 minutes. Cells were fixed with 4% paraformaldehyde and subjected for confocal microscopy analysis. All experiments were repeated for at least three times with similar results.</p>