Survival of <i>Fasciola hepatica</i> newly excysted juveniles (NEJs) in <i>Fasciola</i> saline (FS) vs RPMI 1640.
Paul McVeigh
Erin M. McCammick
Paul McCusker
Russell M. Morphew
Angela Mousley
Abbas Abidi
Khalid M. Saifullah
Raman Muthusamy
Ravikumar Gopalakrishnan
Terry W. Spithill
John P. Dalton
Peter M. Brophy
Nikki J. Marks
Aaron G. Maule
10.1371/journal.pntd.0003185.g001
https://plos.figshare.com/articles/figure/_Survival_of_Fasciola_hepatica_newly_excysted_juveniles_NEJs_in_Fasciola_saline_FS_vs_RPMI_1640_/1182374
<p>NEJ viability in FS is improved both by regular media changes (−change = without media changes; +change = with media changes), or by maintenance in larger volumes (50 µl vs 1000 µl). Experiments were replicated 3 times, employing 15 worms per treatment. Symbols represent mean±SEM.</p>
2014-09-25 03:33:58
RNA interference
virulence genes
control target validation
27 nt
RNAi penetrance
dsRNA exposure
RNAi persistence
transcriptional suppression
Fhe σGST
anthelmintic resistance
Juvenile Fasciola spp
protein knockdown dynamics
Cysteine proteases
4 h exposure
probe RNAi dynamics
Gene function
Vitro BackgroundFasciola spp
cathepsin L
Fasciola gigantica juveniles
liver fluke NEJs
Fhe σGST FindingsRobust transcriptional
Vivo Studies
knockdown dynamics
siRNA triggers
protein suppression
liver fluke
Gene silencing
RNAi Dynamics
standardised RNAi protocols
Fasciola hepatica
FheCatL protein suppression
Liver Flukes
21 days
liver fluke cause