Survival of <i>Fasciola hepatica</i> newly excysted juveniles (NEJs) in <i>Fasciola</i> saline (FS) vs RPMI 1640. Paul McVeigh Erin M. McCammick Paul McCusker Russell M. Morphew Angela Mousley Abbas Abidi Khalid M. Saifullah Raman Muthusamy Ravikumar Gopalakrishnan Terry W. Spithill John P. Dalton Peter M. Brophy Nikki J. Marks Aaron G. Maule 10.1371/journal.pntd.0003185.g001 https://plos.figshare.com/articles/figure/_Survival_of_Fasciola_hepatica_newly_excysted_juveniles_NEJs_in_Fasciola_saline_FS_vs_RPMI_1640_/1182374 <p>NEJ viability in FS is improved both by regular media changes (−change = without media changes; +change = with media changes), or by maintenance in larger volumes (50 µl vs 1000 µl). Experiments were replicated 3 times, employing 15 worms per treatment. Symbols represent mean±SEM.</p> 2014-09-25 03:33:58 RNA interference virulence genes control target validation 27 nt RNAi penetrance dsRNA exposure RNAi persistence transcriptional suppression Fhe σGST anthelmintic resistance Juvenile Fasciola spp protein knockdown dynamics Cysteine proteases 4 h exposure probe RNAi dynamics Gene function Vitro BackgroundFasciola spp cathepsin L Fasciola gigantica juveniles liver fluke NEJs Fhe σGST FindingsRobust transcriptional Vivo Studies knockdown dynamics siRNA triggers protein suppression liver fluke Gene silencing RNAi Dynamics standardised RNAi protocols Fasciola hepatica FheCatL protein suppression Liver Flukes 21 days liver fluke cause