A20 Controls Macrophage to Elicit Potent Cytotoxic CD4<sup>+</sup> T Cell Response Lifeng Wang Bangxing Hong Xiaoxia Jiang Lindsey Jones Si-Yi Chen Xue F. Huang 10.1371/journal.pone.0048930 https://plos.figshare.com/articles/dataset/A20_Controls_Macrophage_to_Elicit_Potent_Cytotoxic_CD4_T_Cell_Response__/117657 <div><p>Emerging evidence indicates that CD4<sup>+</sup> T cells possess cytotoxic potential for tumor eradication and perforin/granzyme-mediated cytotoxicity functions as one of the important mechanisms for CD4<sup>+</sup> T cell-triggered cell killing. However, the critical issue is how the cytotoxic CD4<sup>+</sup> T cells are developed. During the course of our work that aims at promoting immunostimulation of APCs by inhibition of negative regulators, we found that A20-silenced Mф drastically induced granzyme B expression in CD4<sup>+</sup> T cells. As a consequence, the granzyme-highly expressing CD4<sup>+</sup> T cells exhibited a strong cytotoxic activity that restricted tumor development. We found that A20-silenced Mф activated cytotoxic CD4<sup>+</sup> T cells by MHC class-II restricted mechanism and the activation was largely dependent on enhanced production of IFN-γ.</p> </div> 2012-11-07 02:07:37 a20 controls macrophage elicit potent cytotoxic response