10.1371/journal.pone.0108138.g001 Kathryn A. Radigan Kathryn A. Radigan Luisa Morales-Nebreda Luisa Morales-Nebreda Saul Soberanes Saul Soberanes Trevor Nicholson Trevor Nicholson Recep Nigdelioglu Recep Nigdelioglu Takugo Cho Takugo Cho Monica Chi Monica Chi Robert B. Hamanaka Robert B. Hamanaka Alexander V. Misharin Alexander V. Misharin Harris Perlman Harris Perlman G. R. Scott Budinger G. R. Scott Budinger Gökhan M. Mutlu Gökhan M. Mutlu Effect of leptin receptor function on the survival, the degree of lung injury and inflammation during influenza A infection. Public Library of Science 2014 influenza mortality leptin receptor leptin receptor deficiency alveolar type II cells infection leptin receptor results Leptin Receptor Deficient Mice H 1N outbreak lung epithelial cells lung injury severity.ResultsThe clearance 2014-09-18 02:43:23 Figure https://plos.figshare.com/articles/figure/_Effect_of_leptin_receptor_function_on_the_survival_the_degree_of_lung_injury_and_inflammation_during_influenza_A_infection_/1173388 <p><i>db/db</i> and C57BL/6 mice were inoculated with influenza A virus (A/WSN/33 [H1N1]) <b>(a)</b> 1500 pfu/mouse or <b>(b)</b> 500 pfu/mouse and mortality was subsequently assessed on a daily basis. Percentage of weight loss was also followed daily for the mice infected with <b>(c)</b> 1500 pfu/mouse or <b>(d)</b> 500 pfu/mouse. Four days after infection with influenza A virus 1500 pfu/mouse, <i>db/db</i> and C57BL/6 mice underwent a bronchoalveolar lavage for <b>(e</b>) assessment of cell count, <b>(f)</b> total protein, <b>(g,h)</b> inflammatory cytokines, <b>(i)</b> flow cytometry, and <b>(j)</b> the assessment of lung pathology (Hematoxylin and Eosin staining). * p<0.05 compared to PBS control. ** p<0.05 <i>db/db</i> compared to wild-type mice.</p>