10.1371/journal.pone.0106597.g005
Svetlana Rabinovich
Svetlana
Rabinovich
Rebecca L. R. Powell
Rebecca
L. R. Powell
Ross W. B. Lindsay
Ross
W. B. Lindsay
Maoli Yuan
Maoli
Yuan
Alexei Carpov
Alexei
Carpov
Aaron Wilson
Aaron
Wilson
Mary Lopez
Mary
Lopez
John W. Coleman
John
W. Coleman
Denise Wagner
Denise
Wagner
Palka Sharma
Palka
Sharma
Marina Kemelman
Marina
Kemelman
Kevin J. Wright
Kevin
J. Wright
John P. Seabrook
John
P. Seabrook
Heather Arendt
Heather
Arendt
Jennifer Martinez
Jennifer
Martinez
Joanne DeStefano
Joanne
DeStefano
Maria J. Chiuchiolo
Maria
J. Chiuchiolo
Christopher L. Parks
Christopher
L. Parks
Effect of IN rVSV-EnvG<sub>4</sub>-G<sub>6</sub> adminstration.
Public Library of Science
2014
G position
Significant quantities
immunogenic HIV
Conformationally Intact
humoral responses
novel vector
cytoplasmic tail regions
surface glycoprotein
G gene
regimen eliciting
Functional HIV
flow cytometry
EnvG functionality
IM VSV
surface EnvG
Env trimers
HIV vaccine platform
clade B Env immunogen
attenuating quantities
SIV challenge
stomatitis virus
growth attenuation
elisa
attenuated HIV
HIV immunogens
Elicits Potent Cellular
Envelope Trimers
Western Blot analysis
surface G
il
rhesus macaques
neutralizing Ab titers
IM electroporated plasmids encoding EnvG
safety concerns
2014-09-12 02:51:56
Figure
https://plos.figshare.com/articles/figure/_Effect_of_IN_rVSV_EnvG_4_G_6_adminstration_/1169556
<p>(a) Mean body weights and temperatures (b) of inoculated mice. (c) Mean copy numbers of VSV N genomic RNA or (d) VSV N mRNA per mg of indicated tissue or mL of blood. Nā=ā4ā12, dependent on study day. Fresh tissue specimens were homogenized, clarified by centrifugation and supernatants were subjected to RNA extraction and qPCR. All samples were tested in duplicate. Dotted lines indicate limits of detection. SEM is shown. *<i>p</i><0.05 for comparison of rVSV-EnvG<sub>4</sub>-G<sub>6</sub> to rVSV-G<sub>4</sub>. All PBS and rVSV-EnvG<sub>4</sub>-ĪG values were found to be significantly lower than rVSV-EnvG<sub>4</sub>-G<sub>6</sub> and rVSV-G<sub>4</sub> values.</p>