10.1371/journal.pone.0106597.g005 Svetlana Rabinovich Svetlana Rabinovich Rebecca L. R. Powell Rebecca L. R. Powell Ross W. B. Lindsay Ross W. B. Lindsay Maoli Yuan Maoli Yuan Alexei Carpov Alexei Carpov Aaron Wilson Aaron Wilson Mary Lopez Mary Lopez John W. Coleman John W. Coleman Denise Wagner Denise Wagner Palka Sharma Palka Sharma Marina Kemelman Marina Kemelman Kevin J. Wright Kevin J. Wright John P. Seabrook John P. Seabrook Heather Arendt Heather Arendt Jennifer Martinez Jennifer Martinez Joanne DeStefano Joanne DeStefano Maria J. Chiuchiolo Maria J. Chiuchiolo Christopher L. Parks Christopher L. Parks Effect of IN rVSV-EnvG<sub>4</sub>-G<sub>6</sub> adminstration. Public Library of Science 2014 G position Significant quantities immunogenic HIV Conformationally Intact humoral responses novel vector cytoplasmic tail regions surface glycoprotein G gene regimen eliciting Functional HIV flow cytometry EnvG functionality IM VSV surface EnvG Env trimers HIV vaccine platform clade B Env immunogen attenuating quantities SIV challenge stomatitis virus growth attenuation elisa attenuated HIV HIV immunogens Elicits Potent Cellular Envelope Trimers Western Blot analysis surface G il rhesus macaques neutralizing Ab titers IM electroporated plasmids encoding EnvG safety concerns 2014-09-12 02:51:56 Figure https://plos.figshare.com/articles/figure/_Effect_of_IN_rVSV_EnvG_4_G_6_adminstration_/1169556 <p>(a) Mean body weights and temperatures (b) of inoculated mice. (c) Mean copy numbers of VSV N genomic RNA or (d) VSV N mRNA per mg of indicated tissue or mL of blood. Nā€Š=ā€Š4ā€“12, dependent on study day. Fresh tissue specimens were homogenized, clarified by centrifugation and supernatants were subjected to RNA extraction and qPCR. All samples were tested in duplicate. Dotted lines indicate limits of detection. SEM is shown. *<i>p</i><0.05 for comparison of rVSV-EnvG<sub>4</sub>-G<sub>6</sub> to rVSV-G<sub>4</sub>. All PBS and rVSV-EnvG<sub>4</sub>-Ī”G values were found to be significantly lower than rVSV-EnvG<sub>4</sub>-G<sub>6</sub> and rVSV-G<sub>4</sub> values.</p>