Fusogenicity and Functionality of EnvG.
Svetlana Rabinovich
Rebecca L. R. Powell
Ross W. B. Lindsay
Maoli Yuan
Alexei Carpov
Aaron Wilson
Mary Lopez
John W. Coleman
Denise Wagner
Palka Sharma
Marina Kemelman
Kevin J. Wright
John P. Seabrook
Heather Arendt
Jennifer Martinez
Joanne DeStefano
Maria J. Chiuchiolo
Christopher L. Parks
10.1371/journal.pone.0106597.g003
https://plos.figshare.com/articles/figure/_Fusogenicity_and_Functionality_of_EnvG_/1169546
<p>(a) 10<sup>7</sup> 293T cells were transfected with pEnvG or empty vector using Mirus Trans-IT 293 according to manufacturer's protocol. 48 h post-transfection, 293T cells were overlaid with 2×10<sup>6</sup> CD4+CCR5+ GHOST cells. 48 h after overlay, cells were visualized under light microscope and images were captured. (b) 10<sup>6</sup> CD4+CCR5+ GHOST cells were infected as above after pre-incubation with anti-VSV-G (Vi10) and/or anti-Env cocktail. After infection, Vi10 and/or anti-Env cocktail was included in the culture media. Eight hours post infection, cells were visualized under light microscope and images were captured.</p>
2014-09-12 02:51:56
G position
Significant quantities
immunogenic HIV
Conformationally Intact
humoral responses
novel vector
cytoplasmic tail regions
surface glycoprotein
G gene
regimen eliciting
Functional HIV
flow cytometry
EnvG functionality
IM VSV
surface EnvG
Env trimers
HIV vaccine platform
clade B Env immunogen
attenuating quantities
SIV challenge
stomatitis virus
growth attenuation
elisa
attenuated HIV
HIV immunogens
Elicits Potent Cellular
Envelope Trimers
Western Blot analysis
surface G
il
rhesus macaques
neutralizing Ab titers
IM electroporated plasmids encoding EnvG
safety concerns