Fusogenicity and Functionality of EnvG. Svetlana Rabinovich Rebecca L. R. Powell Ross W. B. Lindsay Maoli Yuan Alexei Carpov Aaron Wilson Mary Lopez John W. Coleman Denise Wagner Palka Sharma Marina Kemelman Kevin J. Wright John P. Seabrook Heather Arendt Jennifer Martinez Joanne DeStefano Maria J. Chiuchiolo Christopher L. Parks 10.1371/journal.pone.0106597.g003 https://plos.figshare.com/articles/figure/_Fusogenicity_and_Functionality_of_EnvG_/1169546 <p>(a) 10<sup>7</sup> 293T cells were transfected with pEnvG or empty vector using Mirus Trans-IT 293 according to manufacturer's protocol. 48 h post-transfection, 293T cells were overlaid with 2×10<sup>6</sup> CD4+CCR5+ GHOST cells. 48 h after overlay, cells were visualized under light microscope and images were captured. (b) 10<sup>6</sup> CD4+CCR5+ GHOST cells were infected as above after pre-incubation with anti-VSV-G (Vi10) and/or anti-Env cocktail. After infection, Vi10 and/or anti-Env cocktail was included in the culture media. Eight hours post infection, cells were visualized under light microscope and images were captured.</p> 2014-09-12 02:51:56 G position Significant quantities immunogenic HIV Conformationally Intact humoral responses novel vector cytoplasmic tail regions surface glycoprotein G gene regimen eliciting Functional HIV flow cytometry EnvG functionality IM VSV surface EnvG Env trimers HIV vaccine platform clade B Env immunogen attenuating quantities SIV challenge stomatitis virus growth attenuation elisa attenuated HIV HIV immunogens Elicits Potent Cellular Envelope Trimers Western Blot analysis surface G il rhesus macaques neutralizing Ab titers IM electroporated plasmids encoding EnvG safety concerns