%0 Figure %A Rabinovich, Svetlana %A L. R. Powell, Rebecca %A W. B. Lindsay, Ross %A Yuan, Maoli %A Carpov, Alexei %A Wilson, Aaron %A Lopez, Mary %A W. Coleman, John %A Wagner, Denise %A Sharma, Palka %A Kemelman, Marina %A J. Wright, Kevin %A P. Seabrook, John %A Arendt, Heather %A Martinez, Jennifer %A DeStefano, Joanne %A J. Chiuchiolo, Maria %A L. Parks, Christopher %D 2014 %T rVSV-EnvG4-G6 characterization. %U https://plos.figshare.com/articles/figure/_rVSV_EnvG_4_G_6_characterization_/1169523 %R 10.1371/journal.pone.0106597.g002 %2 https://plos.figshare.com/ndownloader/files/1675098 %K G position %K Significant quantities %K immunogenic HIV %K Conformationally Intact %K humoral responses %K novel vector %K cytoplasmic tail regions %K surface glycoprotein %K G gene %K regimen eliciting %K Functional HIV %K flow cytometry %K EnvG functionality %K IM VSV %K surface EnvG %K Env trimers %K HIV vaccine platform %K clade B Env immunogen %K attenuating quantities %K SIV challenge %K stomatitis virus %K growth attenuation %K elisa %K attenuated HIV %K HIV immunogens %K Elicits Potent Cellular %K Envelope Trimers %K Western Blot analysis %K surface G %K il %K rhesus macaques %K neutralizing Ab titers %K IM electroporated plasmids encoding EnvG %K safety concerns %X

(a) After a 24 hr infection, total infected Vero cell lysates were collected and proteins were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE). Blot was probed with anti-VSV-N and anti-VSV-GIN CT, which does not recognize GNJ. IN: rVSV-EnvG4-G6IN; NJ: rVSV-EnvG4-G6NJ. (b-d) Sucrose-gradient purified rVSV-EnvG4-G6 particles were separated by SDS-PAGE. (b) Blots of 106 pfu of rVSV-EnvG4-G6IN and 2.5×106 pfu rVSV-EnvG4-G6NJ vectors were probed separately for EnvG using anti-gp120. (c) Blot of 106 pfu rVSV-EnvG4-G6IN was probed with anti-VSV-GIN CT. (d) Blot of 106 pfu rVSV-EnvG4-G6IN and rVSV-EnvG4-G6NJ was probed with anti-VSV-G. (e) Replication kinetics of rVSV-EnvG4-G6 and rVSV-G4 viruses in Vero cells. Vero cells were infected in 6-well plates at an MOI of 0.1. At various intervals post infection, supernatant was collected from duplicate wells and virus was titrated. Plaques were counted manually after cell fixation.

%I PLOS ONE