%0 Figure %A Rabinovich, Svetlana %A L. R. Powell, Rebecca %A W. B. Lindsay, Ross %A Yuan, Maoli %A Carpov, Alexei %A Wilson, Aaron %A Lopez, Mary %A W. Coleman, John %A Wagner, Denise %A Sharma, Palka %A Kemelman, Marina %A J. Wright, Kevin %A P. Seabrook, John %A Arendt, Heather %A Martinez, Jennifer %A DeStefano, Joanne %A J. Chiuchiolo, Maria %A L. Parks, Christopher %D 2014 %T Genetic layout of the rVSV-EnvG4-G6 vector, HIV-1 Env, and the EnvG insert. %U https://plos.figshare.com/articles/figure/_Genetic_layout_of_the_rVSV_EnvG_4_G_6_vector_HIV_1_Env_and_the_EnvG_insert_/1169519 %R 10.1371/journal.pone.0106597.g001 %2 https://plos.figshare.com/ndownloader/files/1675091 %K G position %K Significant quantities %K immunogenic HIV %K Conformationally Intact %K humoral responses %K novel vector %K cytoplasmic tail regions %K surface glycoprotein %K G gene %K regimen eliciting %K Functional HIV %K flow cytometry %K EnvG functionality %K IM VSV %K surface EnvG %K Env trimers %K HIV vaccine platform %K clade B Env immunogen %K attenuating quantities %K SIV challenge %K stomatitis virus %K growth attenuation %K elisa %K attenuated HIV %K HIV immunogens %K Elicits Potent Cellular %K Envelope Trimers %K Western Blot analysis %K surface G %K il %K rhesus macaques %K neutralizing Ab titers %K IM electroporated plasmids encoding EnvG %K safety concerns %X

VSV genes are shown in the 3′ to 5′ orientation as ordered on the recombinant genomic VSV plasmid and are not to scale. Arrows below each VSV gene depict the diminishing 3′-to-5′ mRNA transcription gradient. ss: signal sequence. MPER: membrane proximal external region. TM: transmembrane domain. CT: cytoplasmic tail domain. Star denotes site of intracellular Env(G) cleavage by furin.

%I PLOS ONE