10.1371/journal.pone.0107016.g004
Guangzhen Hu
Guangzhen
Hu
Zhenkun Lou
Zhenkun
Lou
Mamta Gupta
Mamta
Gupta
GAS5 suppresses c-Myc translation through direct binding with its mRNA.
Public Library of Science
2014
RNA binding motifs
eIF 4E protein
lymphoma cell lines
translation regulation
GAS 5 RNA
gas 5 siRNA
Protein translation
lncRNA GAS 5
eIF 4E
GAS 5 lncRNA
translation initiation machinery
mrna
Eukaryotic Translation Initiation Factor 4 E
GAS 5
2014-09-08 03:11:01
Figure
https://plos.figshare.com/articles/figure/_GAS5_suppresses_c_Myc_translation_through_direct_binding_with_its_mRNA_/1165024
<p>(<b>A</b>) The protein level of c-Myc was assessed by western blot after the cells were treated with GAS5 or control siRNA followed by the treatment of 100 µg/ml CHX for 1 and 2 hours. (<b>B</b>) c-Myc mRNA level associated with the polysome and non-polysome fractions of GAS5 and control siRNA transfected HEK-293T cells were evaluated by Q-PCR. Data was normalized with total mRNA (non-polysome + polysome). Bars represent mean ±SD from 3 replicates. (<b>C</b>) RNA from HEK-293T cells was pulled down by biotin-labeled GAS5 antisense DNA oligo with GAS5 sense DNA oligo as a control. The binding of c-Myc mRNA to GAS5 was evaluated by RT-PCR, in which GAPDH was used as a control.</p>