Hu, Guangzhen Lou, Zhenkun Gupta, Mamta GAS5 binds to eIF4E through RNA binding motifs. <p>(<b>A</b>) RNA binding motifs, which are italic and bold, in the eIF4E protein were predicted with 2 web-based tools, BindN and PPRInt. The motif, W56, W102 and E103 for m7G binding is bold and underlined. N-terminally located sequence is motif-1 and the C-terminal one is motif-2. (<b>B-C</b>) GAS5 RNA was detected by RT-PCR after RNA-IP assay using HA antibody in the cells transfected with (<b>B</b>) RNA binding deletion mutants (HA-eIF4E<sup>Del1</sup>, HA-eIF4E<sup>Del2</sup> and HA-eIF4E<sup>Del1&2)</sup> and (<b>C</b>) cap binding mutant (HA-eIF4E<sup>cap mutant</sup>). GAPDH was used as a control.</p> RNA binding motifs;eIF 4E protein;lymphoma cell lines;translation regulation;GAS 5 RNA;gas 5 siRNA;Protein translation;lncRNA GAS 5;eIF 4E;GAS 5 lncRNA;translation initiation machinery;mrna;Eukaryotic Translation Initiation Factor 4 E;GAS 5 2014-09-08
    https://plos.figshare.com/articles/figure/_GAS5_binds_to_eIF4E_through_RNA_binding_motifs_/1165022
10.1371/journal.pone.0107016.g002