%0 Figure %A Yadav, Sunita %A Gupta, Smita %A Selvaraj, Chandrabose %A Kumar Doharey, Pawan %A Verma, Anita %A Kumar Singh, Sanjeev %A Saxena, Jitendra Kumar %D 2014 %T BmCRT prevent C1q-dependent haemolysis of Ig-sensitized erythrocytes. %U https://plos.figshare.com/articles/figure/_BmCRT_prevent_C1q_dependent_haemolysis_of_Ig_sensitized_erythrocytes_/1159721 %R 10.1371/journal.pone.0106413.g005 %2 https://plos.figshare.com/ndownloader/files/1659769 %K BmCRT %K Brugia malayi Calreticulin %K CRP binding sites %K crt %K C 1q interaction %K component C 1q %K host complement activation %K Brugia malayi Immunomodulatory Protein Calreticulin %K C 1q %K elisa %K Human C 1q Filarial parasites %K complement pathway activation %K C 1qB chain %K C 1r Molecular dynamics studies %X

Recombinant BmCRT inhibits C1q-dependent haemolysis while BSA (control) shows no effect on this when incubated with C1q deficient serum together with C1q as described in material and method section. Percentage of cell lysis was calculated with reference to 100% lysis of the cells in water. The significant were analyzed by two-way ANOVA (***P<0.001). Assay was performed in triplicates. Bar represent the standard deviations of the mean.

%I PLOS ONE