Purification of recombinant <i>Y. pestis</i> MEP synthase. HaymondAmanda JohnyChinchu DowdyTyrone SchweibenzBrandon VillarroelKaren YoungRichard J. MantoothClark PatelTrishal BasesJessica San JoseGeraldine R. JacksonEmily DowdCynthia S. CouchRobin D. 2014 <p>A Coomassie stained SDS-PAGE shows two lanes of purified His-tagged MEP synthase alongside a molecular weight marker (MW). His-tagged MEP synthase has a predicted molecular weight of 46.7 kDa. The typical yield of purified protein averaged 30 mg per 1 L shake flask.</p>