Dose-response plot of <i>Y. pestis</i> growth as a function of FR900098 concentration. Amanda Haymond Chinchu Johny Tyrone Dowdy Brandon Schweibenz Karen Villarroel Richard Young Clark J. Mantooth Trishal Patel Jessica Bases Geraldine San Jose Emily R. Jackson Cynthia S. Dowd Robin D. Couch 10.1371/journal.pone.0106243.g002 https://plos.figshare.com/articles/figure/_Dose_response_plot_of_Y_pestis_growth_as_a_function_of_FR900098_concentration_/1155772 <p>Fractional growth is calculated as the ratio of cell density (OD<sub>600</sub>) in the presence of inhibitor to cell density in the absence of inhibitor. Nonlinear regression fitting was performed, resulting in an IC<sub>50</sub> of 29 µM (6.4 µg/mL). The goodness-of-fit (R<sup>2</sup>) value is indicated. For comparison, the IC<sub>50</sub> of ampicillin is 10.8 µM (3.8 µg/mL; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106243#pone.0106243.s001" target="_blank">Figure S1</a>).</p> 2014-08-29 02:58:51 KMNADPH product allosteric affector FR 900098 KMDXP drug development DXP binding sites IC 50 values MEP pathway methylerythritol phosphate pestis MEP synthase pilot scale library nadph MEP synthase nm novel antibiotic development