10.1371/journal.pone.0051994 Kimberly M. Jeckel Kimberly M. Jeckel D. N. Rao Veeramachaneni D. N. Rao Veeramachaneni Adam J. Chicco Adam J. Chicco Phillip L. Chapman Phillip L. Chapman Christopher M. Mulligan Christopher M. Mulligan Jennifer R. Hegarty Jennifer R. Hegarty Michael J. Pagliassotti Michael J. Pagliassotti Lindsay A. Ferguson Lindsay A. Ferguson Gerrit J. Bouma Gerrit J. Bouma Melinda A. Frye Melinda A. Frye Docosahexaenoic Acid Supplementation Does Not Improve Western Diet-Induced Cardiomyopathy in Rats Public Library of Science 2012 docosahexaenoic supplementation diet-induced cardiomyopathy rats 2012-12-26 01:28:12 Dataset https://plos.figshare.com/articles/dataset/Docosahexaenoic_Acid_Supplementation_Does_Not_Improve_Western_Diet_Induced_Cardiomyopathy_in_Rats__/115292 <div><p>Obesity increases risk for cardiomyopathy in the absence of hypertension, diabetes or ischemia. The fatty acid milieu, modulated by diet, may modify myocardial structure and function, lending partial explanation for the array of cardiomyopathic phenotypy. We sought to identify gross, cellular and ultrastructural myocardial changes associated with Western diet intake, and subsequent modification with docosahexaenoic acid (DHA) supplementation. Wistar and Sprague-Dawley (SD) rats received 1 of 3 diets: control (CON); Western (WES); Western + DHA (WES+DHA). After 12 weeks of treatment, echocardiography was performed and myocardial adiponectin, fatty acids, collagen, area occupied by lipid and myocytes, and ultrastructure were determined. Strain effects included higher serum adiponectin in Wistar rats, and differences in myocardial fatty acid composition. Diet effects were evident in that both WES and WES+DHA feeding were associated with similarly increased left ventricular (LV) diastolic cranial wall thickness (LVW<sub>cr/d</sub>) and decreased diastolic internal diameter (LVID<sub>d</sub>), compared to CON. Unexpectedly, WES+DHA feeding was associated additionally with increased thickness of the LV cranial wall during systole (LVW<sub>cr/s</sub>) and the caudal wall during diastole (LVW<sub>ca/d</sub>) compared to CON; this was observed concomitantly with increased serum and myocardial adiponectin. Diastolic dysfunction was present in WES+DHA rats compared to both WES and CON. Myocyte cross sectional area (CSA) was greater in WES compared to CON rats. In both fat-fed groups, transmission electron microscopy (TEM) revealed myofibril degeneration, disorganized mitochondrial cristae, lipid inclusions and vacuolation. In the absence of hypertension and whole body insulin resistance, WES+DHA intake was associated with more global LV thickening and with diastolic dysfunction, compared to WES feeding alone. Myocyte hypertrophy, possibly related to subcellular injury, is an early change that may contribute to gross hypertrophy. Strain differences in adipokines and myocardial fatty acid accretion may underlie heterogeneous data from rodent studies.</p> </div>