Schematic representation of the +1-<i>peuA</i>′-<i>flag</i> (A) and +39-<i>peuA</i>′-<i>flag</i> (B) DNA fragments. TanabeTomotaka KatoAyaka ShiuchiKeiichi MiyamotoKatsushiro TsujiboHiroshi MakiJun YamamotoShigeo FunahashiTatsuya 2014 <p>Each of these DNA fragments includes a nucleotide sequence corresponding to the <i>peuA</i> 5′-UTR from the +1 or +39 sites and the nucleotide sequence for the N-terminal 99 amino acid residues (in gray), in addition to a T7 promoter and a FLAG tag preceding the stop codon (TAA). The secondary structures of the 5′-UTRs of the +1 transcript (A) and the +39 transcript (B) of <i>peuA</i> are shown, both of which were predicted by the CentroidFold software (<a href="http://www.ncrna.org/centroidfold/" target="_blank">http://www.ncrna.org/centroidfold/</a>). The RBS and start codon of <i>peuA</i> mRNA are boxed in the secondary structures.</p>