Schematic representation of the +1-<i>peuA</i>′-<i>flag</i> (A) and +39-<i>peuA</i>′-<i>flag</i> (B) DNA fragments. Tomotaka Tanabe Ayaka Kato Keiichi Shiuchi Katsushiro Miyamoto Hiroshi Tsujibo Jun Maki Shigeo Yamamoto Tatsuya Funahashi 10.1371/journal.pone.0105749.g007 https://plos.figshare.com/articles/figure/_Schematic_representation_of_the_1_peuA_flag_A_and_39_peuA_flag_B_DNA_fragments_/1149418 <p>Each of these DNA fragments includes a nucleotide sequence corresponding to the <i>peuA</i> 5′-UTR from the +1 or +39 sites and the nucleotide sequence for the N-terminal 99 amino acid residues (in gray), in addition to a T7 promoter and a FLAG tag preceding the stop codon (TAA). The secondary structures of the 5′-UTRs of the +1 transcript (A) and the +39 transcript (B) of <i>peuA</i> are shown, both of which were predicted by the CentroidFold software (<a href="http://www.ncrna.org/centroidfold/" target="_blank">http://www.ncrna.org/centroidfold/</a>). The RBS and start codon of <i>peuA</i> mRNA are boxed in the secondary structures.</p> 2014-08-22 03:34:55 pH 8.0 pH 7.0 translation stage PeuA complementation experiments Secondary structure prediction Vibrio parahaemolyticus peuA Gene Encoding silico analyses Primer extension analysis Vibrio parahaemolyticus ferric uptake regulator TonB 2 system genes translation analysis deletion mutants peuRS deletion novel gene regulation mechanism enterobactin utilization system operon undescribed receptor peuA expression site vpa transcript Translation initiation Alternative Ferric Enterobactin Receptor system PeuRS