Schematic representation of the +1-<i>peuA</i>′-<i>flag</i> (A) and +39-<i>peuA</i>′-<i>flag</i> (B) DNA fragments.
Tomotaka Tanabe
Ayaka Kato
Keiichi Shiuchi
Katsushiro Miyamoto
Hiroshi Tsujibo
Jun Maki
Shigeo Yamamoto
Tatsuya Funahashi
10.1371/journal.pone.0105749.g007
https://plos.figshare.com/articles/figure/_Schematic_representation_of_the_1_peuA_flag_A_and_39_peuA_flag_B_DNA_fragments_/1149418
<p>Each of these DNA fragments includes a nucleotide sequence corresponding to the <i>peuA</i> 5′-UTR from the +1 or +39 sites and the nucleotide sequence for the N-terminal 99 amino acid residues (in gray), in addition to a T7 promoter and a FLAG tag preceding the stop codon (TAA). The secondary structures of the 5′-UTRs of the +1 transcript (A) and the +39 transcript (B) of <i>peuA</i> are shown, both of which were predicted by the CentroidFold software (<a href="http://www.ncrna.org/centroidfold/" target="_blank">http://www.ncrna.org/centroidfold/</a>). The RBS and start codon of <i>peuA</i> mRNA are boxed in the secondary structures.</p>
2014-08-22 03:34:55
pH 8.0
pH 7.0
translation stage
PeuA
complementation experiments
Secondary structure prediction
Vibrio parahaemolyticus peuA Gene Encoding
silico analyses
Primer extension analysis
Vibrio parahaemolyticus
ferric uptake regulator
TonB 2 system genes
translation analysis
deletion mutants
peuRS deletion
novel gene regulation mechanism
enterobactin utilization
system operon
undescribed receptor
peuA expression
site
vpa
transcript
Translation initiation
Alternative Ferric Enterobactin Receptor
system PeuRS