Tanabe, Tomotaka Kato, Ayaka Shiuchi, Keiichi Miyamoto, Katsushiro Tsujibo, Hiroshi Maki, Jun Yamamoto, Shigeo Funahashi, Tatsuya Primer extension analyses of total RNA from VPD54 or VPD102 to determine the transcription start sites of <i>peuR</i> (A) and <i>peuA</i> (B and C). <p>Total RNAs were isolated from VPD54 (<i>vctA</i>- and <i>irgA</i>-deficient mutant derived from VPD5) and VPD102 (<i>peuRS</i>-deficient mutant derived from VPD54) grown at pH 7.0 and 8.0 in LB-Tris, LB-Tris/+EDDA, and LB-Tris/+EDDA/+Ent media. The amounts of total RNA and primers used for reverse transcription were as follows: (A) 10 µg VppeuR-PE, (B) 10 µg VppeuA-PE, and (C) 150 µg VppeuA-PE. The same primers used for primer extension analysis were used to generate the sequence ladders (A, C, G, T). The transcription start sites and putative Fur boxes are indicated at the top of panels A and B (also see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105749#pone-0105749-g003" target="_blank">Figure 3A</a>).</p> pH 8.0;pH 7.0;translation stage;PeuA;complementation experiments;Secondary structure prediction;Vibrio parahaemolyticus peuA Gene Encoding;silico analyses;Primer extension analysis;Vibrio parahaemolyticus;ferric uptake regulator;TonB 2 system genes;translation analysis;deletion mutants;peuRS deletion;novel gene regulation mechanism;enterobactin utilization;system operon;undescribed receptor;peuA expression;site;vpa;transcript;Translation initiation;Alternative Ferric Enterobactin Receptor;system PeuRS 2014-08-22
    https://plos.figshare.com/articles/figure/_Primer_extension_analyses_of_total_RNA_from_VPD54_or_VPD102_to_determine_the_transcription_start_sites_of_peuR_A_and_peuA_B_and_C_/1149416
10.1371/journal.pone.0105749.g005