Tanabe, Tomotaka Kato, Ayaka Shiuchi, Keiichi Miyamoto, Katsushiro Tsujibo, Hiroshi Maki, Jun Yamamoto, Shigeo Funahashi, Tatsuya SDS-PAGE analysis of Sarkosyl-insoluble OMPs of <i>V. parahaemolyticus</i>. <p>SDS-PAGE analysis was performed with VPD5, VPD107 (seven iron-repressible OMRs-deficient mutant derived from VPD5), VPD108 (<i>peuA</i>-deficient mutant derived from VPD107), VPD108/pRK415-peuA, VPD109 (<i>peuRS</i>-deficient mutant derived from VPD107), and VPD109/pRK415-peuRS. The OMP fractions were prepared from cells grown in LB-Tris medium at pH 7.0, LB-Tris/+EDDA media at pH 7.0 and 8.0, or LB-Tris/+EDDA/+Ent media at pH 7.0 and 8.0. Lanes 1–7 and 9–10 were loaded with 20 µg OMPs, and lane 8 was loaded with 3 µg OMPs. Electrophoresis was performed on 7.5% SDS-polyacrylamide gels (130 mm long) at a constant current of 15 mA at 4°C. The gel was stained with Coomassie Brilliant Blue. The figure shows only the relevant portions of the gel. The iron-repressible OMPs expressed by VPD5 at pH 7.0 under iron-limiting conditions are boxed in lane 2. Lane M, molecular weight marker proteins; closed arrowheads, PeuA.</p> pH 8.0;pH 7.0;translation stage;PeuA;complementation experiments;Secondary structure prediction;Vibrio parahaemolyticus peuA Gene Encoding;silico analyses;Primer extension analysis;Vibrio parahaemolyticus;ferric uptake regulator;TonB 2 system genes;translation analysis;deletion mutants;peuRS deletion;novel gene regulation mechanism;enterobactin utilization;system operon;undescribed receptor;peuA expression;site;vpa;transcript;Translation initiation;Alternative Ferric Enterobactin Receptor;system PeuRS 2014-08-22
    https://plos.figshare.com/articles/figure/_SDS_PAGE_analysis_of_Sarkosyl_insoluble_OMPs_of_V_parahaemolyticus_/1149411
10.1371/journal.pone.0105749.g003