%0 Figure %A Tanabe, Tomotaka %A Kato, Ayaka %A Shiuchi, Keiichi %A Miyamoto, Katsushiro %A Tsujibo, Hiroshi %A Maki, Jun %A Yamamoto, Shigeo %A Funahashi, Tatsuya %D 2014 %T SDS-PAGE analysis of Sarkosyl-insoluble OMPs of V. parahaemolyticus. %U https://plos.figshare.com/articles/figure/_SDS_PAGE_analysis_of_Sarkosyl_insoluble_OMPs_of_V_parahaemolyticus_/1149411 %R 10.1371/journal.pone.0105749.g003 %2 https://plos.figshare.com/ndownloader/files/1647685 %K pH 8.0 %K pH 7.0 %K translation stage %K PeuA %K complementation experiments %K Secondary structure prediction %K Vibrio parahaemolyticus peuA Gene Encoding %K silico analyses %K Primer extension analysis %K Vibrio parahaemolyticus %K ferric uptake regulator %K TonB 2 system genes %K translation analysis %K deletion mutants %K peuRS deletion %K novel gene regulation mechanism %K enterobactin utilization %K system operon %K undescribed receptor %K peuA expression %K site %K vpa %K transcript %K Translation initiation %K Alternative Ferric Enterobactin Receptor %K system PeuRS %X

SDS-PAGE analysis was performed with VPD5, VPD107 (seven iron-repressible OMRs-deficient mutant derived from VPD5), VPD108 (peuA-deficient mutant derived from VPD107), VPD108/pRK415-peuA, VPD109 (peuRS-deficient mutant derived from VPD107), and VPD109/pRK415-peuRS. The OMP fractions were prepared from cells grown in LB-Tris medium at pH 7.0, LB-Tris/+EDDA media at pH 7.0 and 8.0, or LB-Tris/+EDDA/+Ent media at pH 7.0 and 8.0. Lanes 1–7 and 9–10 were loaded with 20 µg OMPs, and lane 8 was loaded with 3 µg OMPs. Electrophoresis was performed on 7.5% SDS-polyacrylamide gels (130 mm long) at a constant current of 15 mA at 4°C. The gel was stained with Coomassie Brilliant Blue. The figure shows only the relevant portions of the gel. The iron-repressible OMPs expressed by VPD5 at pH 7.0 under iron-limiting conditions are boxed in lane 2. Lane M, molecular weight marker proteins; closed arrowheads, PeuA.

%I PLOS ONE