Sathiyamoorthy, Karthik Jiang, Jiansen Xiong Hu, Yao L. Rowe, Cynthia S. Möhl, Britta Chen, Jia Jiang, Wei D. Mellins, Elizabeth Longnecker, Richard Zhou, Z. Hong S. Jardetzky, Theodore BioLayer interferometry (BLI) binding studies with wildtype gp42. <p>Binding kinetics were measured with the Octet RED96 instrument (ForteBio, Pall Corporation) using wildtype (wt) gp42 protein. (A) Biotinylated EBV gHgL was immobilized on streptavidin (SA) biosensor tips and incubated over a range of concentrations (0.4–100 nM) of soluble wt gp42. (B) Biotinylated HLA-DQ2 (CLIP1) was immobilized on streptavidin biosensor tips and incubated over a range of concentrations (2–1200 nM) of soluble wt gp42. (C) Immobilized HLA-DQ2 (CLIP1) was incubated with increasing concentrations of preformed gHgL/gp42 complexes (30–800 nM). Data was fit globally to different binding schemes corresponding to a 1∶1 langmuir binding isotherm (A) and a 2∶1 heterogeneous ligand binding model (B and C). (D) Steady state analysis of the gHgL/gp42 binding to HLA-DQ2 shown in C for equilibrium K<sub>D</sub> determination. Fitted kinetic and equilibrium binding constants are collected in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004309#ppat-1004309-t002" target="_blank">Table 2</a>.</p> gp;membrane fusion;reconstituted B cell entry;Human leukocyte antigen;EBV B Cell Entry;targets EBV infection;class II molecules;membrane bilayers;ghgl;b cells;dna;EBV B cell fusion;membrane fusion activation;HLA class II 2014-08-21
    https://plos.figshare.com/articles/figure/_BioLayer_interferometry_BLI_binding_studies_with_wildtype_gp42_/1147808
10.1371/journal.ppat.1004309.g002