10.1371/journal.pone.0105171.g002
Elien Gevaert
Elien
Gevaert
Laurent Dollé
Laurent
Dollé
Thomas Billiet
Thomas
Billiet
Peter Dubruel
Peter
Dubruel
Leo van Grunsven
Leo
van Grunsven
Aart van Apeldoorn
Aart
van Apeldoorn
Ria Cornelissen
Ria
Cornelissen
Evaluation of hepatocyte phenotype for different micro-aggregate sizes.
Public Library of Science
2014
hepatocyte phenotype
hepatic tissue engineering
aggregate
2014-08-18 03:33:18
Figure
https://plos.figshare.com/articles/figure/_Evaluation_of_hepatocyte_phenotype_for_different_micro_aggregate_sizes_/1143194
<p>(<b>A</b>) Gene expression of albumin. Gene expression levels of albumin were determined for different micro-aggregation conditions and compared to cells cultured on tissue culture plastic (TCP). (<b>B</b>) Albumin secretion at day 3 after isolation for different micro-aggregation conditions. <i>Data are mean ± SD, n = 3, **p<0,01; compared to cells cultivated as monolayers (TCP).</i> (<b>C</b>) Gene expression of CypA3. Gene expression levels of Cyp3A for different aggregate dimensions compared to cells cultured on tissue culture plates (TCP). Gene expression of Cyp3A was determined after 72 hours of cultivation in the micro-wells. <i>Data are mean RQ ± SD, n = 3. *p<0.05; **p<0.01</i> (<b>D</b>) Induced cytochrome 3A4 activity in aggregates of different diameter versus cells cultured on tissue culture plastic. Cyp3A4 activity was quantified after treatment with an inducing agent (hydrocortisone) using a luciferase based assay after 3 days of isolation. <i>Data are mean ± SEM, n = 3. *p<0,05; **p<0,01; ***p<0,001 compared to cells cultured as monolayers (TCP)</i>. (<b>E–F</b>) Live/dead stain of micro-aggregates with diameters of 200 µm (E) and 100 µm (F). Dead cells are stained red, while viable cells are stained green. <i>scale bar = 50 </i><i>µm</i>.</p>