10.1371/journal.ppat.1008233.g007
Julio C. Ayala
Julio C.
Ayala
William M. Shafer
William M.
Shafer
Effect of different carbon sources on <i>lctP</i> expression.
Public Library of Science
2019
hydrogen peroxide
TSS
FA
gdhR
GdhR regulon
gonococcal gene encoding
H 2 O 2
lctP expression
L-lactate
2019-12-20 18:32:33
Figure
https://plos.figshare.com/articles/figure/Effect_of_different_carbon_sources_on_i_lctP_i_expression_/11420706
<p>FA19 reporter strain JC28 and isogenic mutant JC29 (<i>gdhR</i>::<i>kan</i>) containing an <i>lctP</i>-<i>lacZ</i> fusion in vector pLES94-<i>lctP</i> were grown to stationary phase on GC broth supplemented with a concentration range (1.38, 2.75 and 5.50 mM) for each carbon source. Titration of glucose supplemented cultures was done with a fix concentration of 3 mM L-lactate, and titration with L-lactate or pyruvate was done with a fix concentration of 1.5 mM glucose. β-galactosidase was expressed from the <i>lctP</i> transcriptional and translational signals and its activity was determined in Miller units. Data are presented as the mean (bar) plus the standard error of the mean (error bar) of 3 biological samples and two technical replicates each. ** represents significant statistical differences at p<0.01 within each carbohydrate titration group as determined by a non-parametric Kruskal-Wallis test and a Dunn’s posttest.</p>