Heat map view of the final 96.96 Dynamic Array run with individual assays on the x axis and individual samples on y axis. Dominika Borowska Richard Kuo Richard A. Bailey Kellie A. Watson Pete Kaiser Lonneke Vervelde Mark P. Stevens 10.1371/journal.pone.0225658.g003 https://plos.figshare.com/articles/figure/Heat_map_view_of_the_final_96_96_Dynamic_Array_run_with_individual_assays_on_the_x_axis_and_individual_samples_on_y_axis_/11312096 <p>The RNA extracted from tissue samples from broilers raised in two different biosecurity environments (sibling-test and pedigree farms); samples from five tissues from four birds on each of the two farms were used; pooled preamplified cDNA was used for standard curve dilutions.</p> 2019-12-03 18:44:20 multiplexed qPCR 96.96 Fluidigm Dynamic Array 6 nanolitre reactions broiler chickens PCR-based platform microfluidic system 96 transcripts transcript levels transcriptome data amplicon sequencing chicken macrophages RNA-seq analysis preamplification steps Conventional qPCR analysis Gut-associated lymphoid tissues blood cells transcriptional response 89 genes immune-related genes biosecurity levels multiplexed qPCR-based platform screen vaccine responses chicken immune-related genes multiplex qPCR array 96 samples analyse high-throughput assays blood leukocytes curve analysis pathogen-specific probes dendritic cells