Heat map view of the final 96.96 Dynamic Array run with individual assays on the x axis and individual samples on y axis.
Dominika Borowska
Richard Kuo
Richard A. Bailey
Kellie A. Watson
Pete Kaiser
Lonneke Vervelde
Mark P. Stevens
10.1371/journal.pone.0225658.g003
https://plos.figshare.com/articles/figure/Heat_map_view_of_the_final_96_96_Dynamic_Array_run_with_individual_assays_on_the_x_axis_and_individual_samples_on_y_axis_/11312096
<p>The RNA extracted from tissue samples from broilers raised in two different biosecurity environments (sibling-test and pedigree farms); samples from five tissues from four birds on each of the two farms were used; pooled preamplified cDNA was used for standard curve dilutions.</p>
2019-12-03 18:44:20
multiplexed qPCR 96.96 Fluidigm Dynamic Array
6 nanolitre reactions
broiler chickens
PCR-based platform
microfluidic system
96 transcripts
transcript levels
transcriptome data
amplicon sequencing
chicken macrophages
RNA-seq analysis
preamplification steps
Conventional qPCR analysis
Gut-associated lymphoid tissues
blood cells
transcriptional response
89 genes
immune-related genes
biosecurity levels
multiplexed qPCR-based platform
screen vaccine responses
chicken immune-related genes
multiplex qPCR array
96 samples
analyse
high-throughput assays
blood leukocytes
curve analysis
pathogen-specific probes
dendritic cells