10.1371/journal.pone.0225658.g002
Dominika Borowska
Dominika
Borowska
Richard Kuo
Richard
Kuo
Richard A. Bailey
Richard A.
Bailey
Kellie A. Watson
Kellie A.
Watson
Pete Kaiser
Pete
Kaiser
Lonneke Vervelde
Lonneke
Vervelde
Mark P. Stevens
Mark P.
Stevens
Validation of 96.96 Dynamic Array.
Public Library of Science
2019
multiplexed qPCR 96.96 Fluidigm Dynamic Array
6 nanolitre reactions
broiler chickens
PCR-based platform
microfluidic system
96 transcripts
transcript levels
transcriptome data
amplicon sequencing
chicken macrophages
RNA-seq analysis
preamplification steps
Conventional qPCR analysis
Gut-associated lymphoid tissues
blood cells
transcriptional response
89 genes
immune-related genes
biosecurity levels
multiplexed qPCR-based platform
screen vaccine responses
chicken immune-related genes
multiplex qPCR array
96 samples
analyse
high-throughput assays
blood leukocytes
curve analysis
pathogen-specific probes
dendritic cells
2019-12-03 18:44:19
Figure
https://plos.figshare.com/articles/figure/Validation_of_96_96_Dynamic_Array_/11312093
<p>A) standard RNA samples of COS-7 cells transfected with plasmids containing genes for: CXCLi2, IL1B, IL12B, IL6, IL18, reverse transcribed and used in limiting dilutions; B) cDNA and cDNA after preamplification showing higher amplification in the later; brighter colour indicates lower Cq value, higher amplification.</p>