Optimisation of primer pairs.
Dominika Borowska
Richard Kuo
Richard A. Bailey
Kellie A. Watson
Pete Kaiser
Lonneke Vervelde
Mark P. Stevens
10.1371/journal.pone.0225658.g001
https://plos.figshare.com/articles/figure/Optimisation_of_primer_pairs_/11312090
<p>Examples of melting curve analysis based on serial dilutions of pooled cDNA generated from tissues/cells isolated from various infection studies.</p>
2019-12-03 18:44:18
multiplexed qPCR 96.96 Fluidigm Dynamic Array
6 nanolitre reactions
broiler chickens
PCR-based platform
microfluidic system
96 transcripts
transcript levels
transcriptome data
amplicon sequencing
chicken macrophages
RNA-seq analysis
preamplification steps
Conventional qPCR analysis
Gut-associated lymphoid tissues
blood cells
transcriptional response
89 genes
immune-related genes
biosecurity levels
multiplexed qPCR-based platform
screen vaccine responses
chicken immune-related genes
multiplex qPCR array
96 samples
analyse
high-throughput assays
blood leukocytes
curve analysis
pathogen-specific probes
dendritic cells