Optimisation of primer pairs. Dominika Borowska Richard Kuo Richard A. Bailey Kellie A. Watson Pete Kaiser Lonneke Vervelde Mark P. Stevens 10.1371/journal.pone.0225658.g001 https://plos.figshare.com/articles/figure/Optimisation_of_primer_pairs_/11312090 <p>Examples of melting curve analysis based on serial dilutions of pooled cDNA generated from tissues/cells isolated from various infection studies.</p> 2019-12-03 18:44:18 multiplexed qPCR 96.96 Fluidigm Dynamic Array 6 nanolitre reactions broiler chickens PCR-based platform microfluidic system 96 transcripts transcript levels transcriptome data amplicon sequencing chicken macrophages RNA-seq analysis preamplification steps Conventional qPCR analysis Gut-associated lymphoid tissues blood cells transcriptional response 89 genes immune-related genes biosecurity levels multiplexed qPCR-based platform screen vaccine responses chicken immune-related genes multiplex qPCR array 96 samples analyse high-throughput assays blood leukocytes curve analysis pathogen-specific probes dendritic cells