10.1371/journal.pone.0225658.g001 Dominika Borowska Dominika Borowska Richard Kuo Richard Kuo Richard A. Bailey Richard A. Bailey Kellie A. Watson Kellie A. Watson Pete Kaiser Pete Kaiser Lonneke Vervelde Lonneke Vervelde Mark P. Stevens Mark P. Stevens Optimisation of primer pairs. Public Library of Science 2019 multiplexed qPCR 96.96 Fluidigm Dynamic Array 6 nanolitre reactions broiler chickens PCR-based platform microfluidic system 96 transcripts transcript levels transcriptome data amplicon sequencing chicken macrophages RNA-seq analysis preamplification steps Conventional qPCR analysis Gut-associated lymphoid tissues blood cells transcriptional response 89 genes immune-related genes biosecurity levels multiplexed qPCR-based platform screen vaccine responses chicken immune-related genes multiplex qPCR array 96 samples analyse high-throughput assays blood leukocytes curve analysis pathogen-specific probes dendritic cells 2019-12-03 18:44:18 Figure https://plos.figshare.com/articles/figure/Optimisation_of_primer_pairs_/11312090 <p>Examples of melting curve analysis based on serial dilutions of pooled cDNA generated from tissues/cells isolated from various infection studies.</p>