10.1371/journal.pone.0104441.g001 Junghwa Lim Junghwa Lim Paul R. Sabandal Paul R. Sabandal Ana Fernandez Ana Fernandez John Martin Sabandal John Martin Sabandal Hyun-Gwan Lee Hyun-Gwan Lee Peter Evans Peter Evans Kyung-An Han Kyung-An Han The sterility phenotype of the <i>octβ2r</i> mutant female is due to defective ovulation. Public Library of Science 2014 anatomy Reproductive system organisms animals invertebrates arthropoda insects drosophila Drosophila melanogaster physiology Reproductive physiology Model organisms Animal models sterility phenotype mutant defective 2014-08-06 03:04:00 Figure https://plos.figshare.com/articles/figure/_The_sterility_phenotype_of_the_oct_946_2r_mutant_female_is_due_to_defective_ovulation_/1129216 <p>(A) Copulation behavior. Virgin wild-type <i>CS</i> or <i>octβ2r</i> females were singly paired with naïve <i>CS</i> males to examine copulation behavior. The <i>CS</i> males paired with <i>octβ2r</i> females exhibited copulation latency and duration comparable to those paired with <i>CS</i> females (<i>p</i>>0.05 by Student’s t-test; <i>n</i> = 17–24). (B) Sperm retention. <i>CS</i> and <i>octβ2r</i> females were mated with <i>dj-GFP</i> males carrying GFP-tagged sperm (green) and the reproductive systems were dissected and counterstained with DAPI (blue). <i>CS</i> and <i>octβ2r</i> females had comparable sperm storage in the seminal receptacle and spermathecae at 24 and 48 h after mating. Spt, spermathecae; SmRcp, seminal receptacle. Scale bar, 50 µm. (C) Ovulation. <i>CS</i> and <i>octβ2r</i> females were mated with <i>CS</i> males and the reproductive systems were dissected to examine the location of the egg in the oviduct or uterus. The <i>octβ2r</i> females had significantly lower levels of ovulation than <i>CS</i> females (***, <i>p</i><0.0001 by Student’s t-tests; <i>n</i> = 10). (D) Courtship behavior. Virgin <i>CS</i> and <i>octβ2r</i> females were singly paired with naïve <i>CS</i> males to measure courtship activity. The mated females were tested again with new naïve males 48 h later to measure courtship receptivity. The percentage of time that the CS males spent courting <i>CS</i> or <i>octβ2r</i> females represents courtship index. With both <i>CS</i> and <i>octβ2r</i> females, courtship activities of the males paired with mated females were significant lower than those of the males paired with virgin females (<i>p</i><0.0001 by Mann-Whitney; <i>n</i> = 32–69). Thus, <i>CS</i> and <i>octβ2r</i> females have comparable pre- and post-mating courtship activity. ns, not significant.</p>