10.1371/journal.pone.0104441.g001
Junghwa Lim
Junghwa
Lim
Paul R. Sabandal
Paul
R. Sabandal
Ana Fernandez
Ana
Fernandez
John Martin Sabandal
John
Martin Sabandal
Hyun-Gwan Lee
Hyun-Gwan
Lee
Peter Evans
Peter
Evans
Kyung-An Han
Kyung-An
Han
The sterility phenotype of the <i>octβ2r</i> mutant female is due to defective ovulation.
Public Library of Science
2014
anatomy
Reproductive system
organisms
animals
invertebrates
arthropoda
insects
drosophila
Drosophila melanogaster
physiology
Reproductive physiology
Model organisms
Animal models
sterility
phenotype
mutant
defective
2014-08-06 03:04:00
Figure
https://plos.figshare.com/articles/figure/_The_sterility_phenotype_of_the_oct_946_2r_mutant_female_is_due_to_defective_ovulation_/1129216
<p>(A) Copulation behavior. Virgin wild-type <i>CS</i> or <i>octβ2r</i> females were singly paired with naïve <i>CS</i> males to examine copulation behavior. The <i>CS</i> males paired with <i>octβ2r</i> females exhibited copulation latency and duration comparable to those paired with <i>CS</i> females (<i>p</i>>0.05 by Student’s t-test; <i>n</i> = 17–24). (B) Sperm retention. <i>CS</i> and <i>octβ2r</i> females were mated with <i>dj-GFP</i> males carrying GFP-tagged sperm (green) and the reproductive systems were dissected and counterstained with DAPI (blue). <i>CS</i> and <i>octβ2r</i> females had comparable sperm storage in the seminal receptacle and spermathecae at 24 and 48 h after mating. Spt, spermathecae; SmRcp, seminal receptacle. Scale bar, 50 µm. (C) Ovulation. <i>CS</i> and <i>octβ2r</i> females were mated with <i>CS</i> males and the reproductive systems were dissected to examine the location of the egg in the oviduct or uterus. The <i>octβ2r</i> females had significantly lower levels of ovulation than <i>CS</i> females (***, <i>p</i><0.0001 by Student’s t-tests; <i>n</i> = 10). (D) Courtship behavior. Virgin <i>CS</i> and <i>octβ2r</i> females were singly paired with naïve <i>CS</i> males to measure courtship activity. The mated females were tested again with new naïve males 48 h later to measure courtship receptivity. The percentage of time that the CS males spent courting <i>CS</i> or <i>octβ2r</i> females represents courtship index. With both <i>CS</i> and <i>octβ2r</i> females, courtship activities of the males paired with mated females were significant lower than those of the males paired with virgin females (<i>p</i><0.0001 by Mann-Whitney; <i>n</i> = 32–69). Thus, <i>CS</i> and <i>octβ2r</i> females have comparable pre- and post-mating courtship activity. ns, not significant.</p>