10.1371/journal.pone.0101797.g005 Lisa Cadavez Lisa Cadavez Joel Montane Joel Montane Gema Alcarraz-Vizán Gema Alcarraz-Vizán Montse Visa Montse Visa Laia Vidal-Fàbrega Laia Vidal-Fàbrega Joan-Marc Servitja Joan-Marc Servitja Anna Novials Anna Novials Endogenous and chemical chaperones decrease ER stress markers in hIAPP-INS1E cells. Public Library of Science 2014 Biochemistry proteins Chaperone proteins cell biology Cell processes Cellular stress responses Endoplasmic reticulum stress response Metabolic disorders Diabetes mellitus type 2 diabetes Precursors of type 2 diabetes Pathology and laboratory medicine Signs and symptoms amyloidosis chaperones er markers hiapp-ins1e 2014-07-10 03:14:57 Figure https://plos.figshare.com/articles/figure/_Endogenous_and_chemical_chaperones_decrease_ER_stress_markers_in_hIAPP_INS1E_cells_/1099575 <p>A) BiP and tubulin levels were determined in hIAPP-INS1E cells previously transduced with Ad-BiP or Ad-GFP for 24 hours. Representative western blotting results showing protein expression levels of CHOP, ATF3, BiP, p-eIF2α and β-actin levels in hIAPP-INS1E cells cultured with B) 0.5 µM Thp for 8 hours or C) with 25 mM of glucose and 400 µM palmitic acid (HG+PA) for 24 hours. Representative results from 3 to 5 individual experiments are shown.</p>