Cadavez, Lisa Montane, Joel Alcarraz-Vizán, Gema Visa, Montse Vidal-Fàbrega, Laia Servitja, Joan-Marc Novials, Anna Increased BiP and PDI expression after adenoviral transduction does not affect cell viability. <p>A) hIAPP-INS1E cells were transduced with different doses (MOI) for 24 hours. Representative Western blotting shows BiP protein levels at indicated doses. B) GFP expression after adenoviral (Ad-PDI/GFP) transduction in hIAPP-INS1E cells for 24 hours. Note an increase in GFP expression that correlates with an increase in MOI. C) Live/death viability assay showing no cell death in hIAPP-INS1E cells transduced for 24 hours with Ad-BiP. Western blotting and images in B and C are representative from 3 independent experiments.</p> Biochemistry;proteins;Chaperone proteins;cell biology;Cell processes;Cellular stress responses;Endoplasmic reticulum stress response;Metabolic disorders;Diabetes mellitus;type 2 diabetes;Precursors of type 2 diabetes;Pathology and laboratory medicine;Signs and symptoms;amyloidosis;bip;pdi;adenoviral;transduction 2014-07-10
    https://plos.figshare.com/articles/figure/_Increased_BiP_and_PDI_expression_after_adenoviral_transduction_does_not_affect_cell_viability_/1099574
10.1371/journal.pone.0101797.g004