%0 Figure %A Cadavez, Lisa %A Montane, Joel %A Alcarraz-Vizán, Gema %A Visa, Montse %A Vidal-Fàbrega, Laia %A Servitja, Joan-Marc %A Novials, Anna %D 2014 %T Increased BiP and PDI expression after adenoviral transduction does not affect cell viability. %U https://plos.figshare.com/articles/figure/_Increased_BiP_and_PDI_expression_after_adenoviral_transduction_does_not_affect_cell_viability_/1099574 %R 10.1371/journal.pone.0101797.g004 %2 https://plos.figshare.com/ndownloader/files/1587859 %K Biochemistry %K proteins %K Chaperone proteins %K cell biology %K Cell processes %K Cellular stress responses %K Endoplasmic reticulum stress response %K Metabolic disorders %K Diabetes mellitus %K type 2 diabetes %K Precursors of type 2 diabetes %K Pathology and laboratory medicine %K Signs and symptoms %K amyloidosis %K bip %K pdi %K adenoviral %K transduction %X
A) hIAPP-INS1E cells were transduced with different doses (MOI) for 24 hours. Representative Western blotting shows BiP protein levels at indicated doses. B) GFP expression after adenoviral (Ad-PDI/GFP) transduction in hIAPP-INS1E cells for 24 hours. Note an increase in GFP expression that correlates with an increase in MOI. C) Live/death viability assay showing no cell death in hIAPP-INS1E cells transduced for 24 hours with Ad-BiP. Western blotting and images in B and C are representative from 3 independent experiments.
%I PLOS ONE