10.1371/journal.pone.0101797.g004 Lisa Cadavez Lisa Cadavez Joel Montane Joel Montane Gema Alcarraz-Vizán Gema Alcarraz-Vizán Montse Visa Montse Visa Laia Vidal-Fàbrega Laia Vidal-Fàbrega Joan-Marc Servitja Joan-Marc Servitja Anna Novials Anna Novials Increased BiP and PDI expression after adenoviral transduction does not affect cell viability. Public Library of Science 2014 Biochemistry proteins Chaperone proteins cell biology Cell processes Cellular stress responses Endoplasmic reticulum stress response Metabolic disorders Diabetes mellitus type 2 diabetes Precursors of type 2 diabetes Pathology and laboratory medicine Signs and symptoms amyloidosis bip pdi adenoviral transduction 2014-07-10 03:14:57 Figure https://plos.figshare.com/articles/figure/_Increased_BiP_and_PDI_expression_after_adenoviral_transduction_does_not_affect_cell_viability_/1099574 <p>A) hIAPP-INS1E cells were transduced with different doses (MOI) for 24 hours. Representative Western blotting shows BiP protein levels at indicated doses. B) GFP expression after adenoviral (Ad-PDI/GFP) transduction in hIAPP-INS1E cells for 24 hours. Note an increase in GFP expression that correlates with an increase in MOI. C) Live/death viability assay showing no cell death in hIAPP-INS1E cells transduced for 24 hours with Ad-BiP. Western blotting and images in B and C are representative from 3 independent experiments.</p>