Hennessy, Emma O'Callaghan, Julie J. Mooij, Marlies Legendre, Claire Camacho-Vanegas, Olga C. Camacho, Sandra Adams, Claire A. Martignetti, John O'Gara, Fergal wtKLF6 knockdown and statin effects in lung epithelial cells. <p>(<b>A</b>) wtKLF6 mRNA expression was reduced by 47.2% compared to vector control (si-luc) cells (<i>P</i> = 0.008). (<b>B</b>) Expression of the wtKLF6 target genes ASAH1, CCL20, iNOS and PPARγ was examined by qRT-PCR in si-wtKLF6 cells compared to vector control. ASAH1, CCL20 and iNOS were inhibited in si-cells compared to vector control cells. PPARγ was unaffected in si-wtKLF6. (<b>C</b>) Simvastatin induced ASAH1 expression but independent of wtKLF6. Simvastatin induced CCL20 (<b>D</b>) and reduced iNOS (<b>E</b>) expression and these appeared to be mediated via wtKLF6. *<i>P</i>, ≤0.05, ** <i>P</i>, ≤0.01.</p> cell biology;Molecular cell biology;genetics;gene expression;immunology;Immune response;inflammation;Immune system;Innate immune system;immunity;Immune activation;microbiology;bacteriology;Gram negative bacteria;Medical microbiology;Microbial pathogens;Bacterial pathogens;Infectious diseases;Bacterial diseases;Pseudomonas infections;Pathology and laboratory medicine;pathogenesis;Host-pathogen interactions;knockdown;statin;epithelial 2014-07-10
    https://plos.figshare.com/articles/figure/_wtKLF6_knockdown_and_statin_effects_in_lung_epithelial_cells_/1099269
10.1371/journal.pone.0102200.g003