Homodimerization of SULT4A1.
Neelima P. Sidharthan
Neville J. Butcher
Deanne J. Mitchell
Rodney F. Minchin
10.1371/journal.pone.0101520.g005
https://plos.figshare.com/articles/figure/_Homodimerization_of_SULT4A1_/1091912
<p>(A) Conservation of the dimerization motif in various human SULTs. The consensus sequence is shown below. (B) Immunoprecipitaton assay for dimerization of SULT4A1 protein. IMR-32 cells were co-transfected with HA-tagged SULT4A1 and either FLAG-tagged wild-type SULT4A1 (WT), mutant SULT4A1 (TV) or empty vector (EV). Isolated cytosolic proteins were immunoprecipitated with anti-HA antibody and detected with anti-FLAG antibody. Western blots of protein lysates prior to immunoprecipitation (Input) demonstrate expression. Western blots using anti-HA antibody (lower panel) demonstrates immunoprecipitation.</p>
2014-07-02 03:04:23
Biochemistry
enzymology
enzymes
biocatalysis
metabolism
cell biology
Molecular cell biology
genetics
gene expression
toxicology
Clinical medicine