Homodimerization of SULT4A1. Neelima P. Sidharthan Neville J. Butcher Deanne J. Mitchell Rodney F. Minchin 10.1371/journal.pone.0101520.g005 https://plos.figshare.com/articles/figure/_Homodimerization_of_SULT4A1_/1091912 <p>(A) Conservation of the dimerization motif in various human SULTs. The consensus sequence is shown below. (B) Immunoprecipitaton assay for dimerization of SULT4A1 protein. IMR-32 cells were co-transfected with HA-tagged SULT4A1 and either FLAG-tagged wild-type SULT4A1 (WT), mutant SULT4A1 (TV) or empty vector (EV). Isolated cytosolic proteins were immunoprecipitated with anti-HA antibody and detected with anti-FLAG antibody. Western blots of protein lysates prior to immunoprecipitation (Input) demonstrate expression. Western blots using anti-HA antibody (lower panel) demonstrates immunoprecipitation.</p> 2014-07-02 03:04:23 Biochemistry enzymology enzymes biocatalysis metabolism cell biology Molecular cell biology genetics gene expression toxicology Clinical medicine